Enrichment of antigen-specific T cells based on activation-dependent CD154 expression and expansion of T-cell lines within 14 days
| Antigen . | Donor ID . | Cell number before stimulation (×106) . | Cell number after CD154 separation (×106) . | Fraction of selected cells, % . | Cell number after 14 d of expansion (×106) . | Expansion factor . |
|---|---|---|---|---|---|---|
| CMV pp65 peptide pool | donor 1 | 52 | 0.79 | 1.52 | 32 | 41 |
| donor 4 | 55 | 0.60 | 1.10 | 35 | 58 | |
| Multi-specific T-cell line (CMV, AdV, EBV, and C albicans) | donor 1 | 52 | 0.61 | 1.17 | 35 | 57 |
| donor 4 | 51.5 | 0.52 | 1.01 | 36 | 69.2 | |
| Multi-specific T-cell line including A fumigatus p41 | donor 2 | 42 | 0.45 | 1.07 | 61 | 136 |
| Antigen . | Donor ID . | Cell number before stimulation (×106) . | Cell number after CD154 separation (×106) . | Fraction of selected cells, % . | Cell number after 14 d of expansion (×106) . | Expansion factor . |
|---|---|---|---|---|---|---|
| CMV pp65 peptide pool | donor 1 | 52 | 0.79 | 1.52 | 32 | 41 |
| donor 4 | 55 | 0.60 | 1.10 | 35 | 58 | |
| Multi-specific T-cell line (CMV, AdV, EBV, and C albicans) | donor 1 | 52 | 0.61 | 1.17 | 35 | 57 |
| donor 4 | 51.5 | 0.52 | 1.01 | 36 | 69.2 | |
| Multi-specific T-cell line including A fumigatus p41 | donor 2 | 42 | 0.45 | 1.07 | 61 | 136 |
Cell numbers are the means of at least 2 independent experiments, except the CMV pp65 single lines.