Table 1 Enrichment of antigen-specific T... | American Society of Hematology

Table 1

Enrichment of antigen-specific T cells based on activation-dependent CD154 expression and expansion of T-cell lines within 14 days

Antigen	Donor ID	Cell number before stimulation (×10⁶)	Cell number after CD154 separation (×10⁶)	Fraction of selected cells, %	Cell number after 14 d of expansion (×10⁶)	Expansion factor
AdV hexon peptide pool	donor 1	51	0.39	0.76	32	82
	donor 2	60	0.45	0.75	33	73
	donor 3	62.5	0.29	0.46	29.5	102
	donor 4	45	0.46	1.02	28	61
	donor 5	56	0.74	1.32	79	107
	donor 6	63	0.72	1.14	13.5	19
	donor 7	43	0.44	1.02	21.1	48
EBV LMP2 peptide pool	donor 1	66.5	0.38	0.56	40.4	108
	donor 2	60	0.36	0.59	20.5	58
	donor 3	62.5	0.53	0.84	22.5	43
	donor 4	59	0.44	0.75	28	64
	donor 5	57	0.76	1.33	39	51
	donor 6	63	0.70	1.11	2.8	4
	donor 7	49	0.60	1.22	58	97
A fumigatus FHT peptide	donor 1	83.5	0.52	0.62	22.5	44
	donor 2	80	0.74	0.92	14.5	20
	donor 3	87.5	0.63	0.71	43.5	70
	donor 4	66	0.31	0.47	1.0	3
	donor 5	48	0.51	1.06	23	45
	donor 6	63	0.77	1.22	3.9	5
	donor 7	52	0.50	0.96	22	44
C albicans MP65 peptide pool	donor 1	55.5	0.19	0.35	24.5	128
	donor 2	60	0.37	0.61	34	93
	donor 3	62.5	0.22	0.35	32	145
	donor 4	55	0.44	0.80	53.5	122
	donor 5	50	0.46	0.92	35	76
	donor 6	59	0.76	1.29	18.5	24
	donor 7	46	0.61	1.33	73	120
Multi-specific T-cell line	donor 1	56	0.52	0.93	35.4	68
	donor 2	75	0.86	1.14	36.5	43
	donor 3	62.5	0.56	0.90	39	70
	donor 4	49	0.73	1.49	22	30
	donor 5	63	0.72	1.14	60	83
	donor 6	59	0.55	0.93	2.7	5
	donor 7	47	0.46	0.98	37	80

Antigen	Donor ID	Cell number before stimulation (×10⁶)	Cell number after CD154 separation (×10⁶)	Fraction of selected cells, %	Cell number after 14 d of expansion (×10⁶)	Expansion factor
AdV hexon peptide pool	donor 1	51	0.39	0.76	32	82
	donor 2	60	0.45	0.75	33	73
	donor 3	62.5	0.29	0.46	29.5	102
	donor 4	45	0.46	1.02	28	61
	donor 5	56	0.74	1.32	79	107
	donor 6	63	0.72	1.14	13.5	19
	donor 7	43	0.44	1.02	21.1	48
EBV LMP2 peptide pool	donor 1	66.5	0.38	0.56	40.4	108
	donor 2	60	0.36	0.59	20.5	58
	donor 3	62.5	0.53	0.84	22.5	43
	donor 4	59	0.44	0.75	28	64
	donor 5	57	0.76	1.33	39	51
	donor 6	63	0.70	1.11	2.8	4
	donor 7	49	0.60	1.22	58	97
A fumigatus FHT peptide	donor 1	83.5	0.52	0.62	22.5	44
	donor 2	80	0.74	0.92	14.5	20
	donor 3	87.5	0.63	0.71	43.5	70
	donor 4	66	0.31	0.47	1.0	3
	donor 5	48	0.51	1.06	23	45
	donor 6	63	0.77	1.22	3.9	5
	donor 7	52	0.50	0.96	22	44
C albicans MP65 peptide pool	donor 1	55.5	0.19	0.35	24.5	128
	donor 2	60	0.37	0.61	34	93
	donor 3	62.5	0.22	0.35	32	145
	donor 4	55	0.44	0.80	53.5	122
	donor 5	50	0.46	0.92	35	76
	donor 6	59	0.76	1.29	18.5	24
	donor 7	46	0.61	1.33	73	120
Multi-specific T-cell line	donor 1	56	0.52	0.93	35.4	68
	donor 2	75	0.86	1.14	36.5	43
	donor 3	62.5	0.56	0.90	39	70
	donor 4	49	0.73	1.49	22	30
	donor 5	63	0.72	1.14	60	83
	donor 6	59	0.55	0.93	2.7	5
	donor 7	47	0.46	0.98	37	80

Cell numbers are the means of at least 2 independent experiments.