Human cell chimerism in the thymus of newborn-engrafted mice at 12 weeks of age
| . | NSG-Tg(hu-mSCF), 0 cGy (N = 6) . | NSG, 0 cGy (N = 6) . | NSG-Tg(hu-mSCF), 100 cGy (N = 7) . | NSG, 100 cGy (N = 10) . |
|---|---|---|---|---|
| Total human leukocytes: CD45+, % | 46.2 ± 34 | 49.8 ± 31 | 62.6 ± 25 | 43.1 ± 33 |
| Thymocyte subsets, % of CD45 | ||||
| CD4+/CD8+ | 64.5 ± 16 | 34.1 ± 31.0 | 28.9 ± 29 | 50.3 ± 34 |
| CD4+ | 15.3 ± 10 | 22.9 ± 27 | 45.4 ± 28 | 22.4 ± 16 |
| CD8+ | 9.1 ± 4.0 | 13.2 ± 11 | 16.0 ± 8.2 | 13.6 ± 13 |
| . | NSG-Tg(hu-mSCF), 0 cGy (N = 6) . | NSG, 0 cGy (N = 6) . | NSG-Tg(hu-mSCF), 100 cGy (N = 7) . | NSG, 100 cGy (N = 10) . |
|---|---|---|---|---|
| Total human leukocytes: CD45+, % | 46.2 ± 34 | 49.8 ± 31 | 62.6 ± 25 | 43.1 ± 33 |
| Thymocyte subsets, % of CD45 | ||||
| CD4+/CD8+ | 64.5 ± 16 | 34.1 ± 31.0 | 28.9 ± 29 | 50.3 ± 34 |
| CD4+ | 15.3 ± 10 | 22.9 ± 27 | 45.4 ± 28 | 22.4 ± 16 |
| CD8+ | 9.1 ± 4.0 | 13.2 ± 11 | 16.0 ± 8.2 | 13.6 ± 13 |
Nonirradiated NSG and NSG-Tg(hu-mSCF), and 100-cGy irradiated NSG and NSG-Tg(hu-mSCF) newborn mice were engrafted with human HSCs as described in “Engraftment of mice with human HSCs.” Mice were analyzed by flow cytometry for the percentage of human CD45+ and the percentage of human CD45+ cells that were positive for specific lineage markers in the thymus at 16-20 weeks of age. Data are mean ± SD. No significant differences were observed.