Human cell chimerism in the blood of newborn-engrafted mice at 12 weeks of age
| Human cell subset . | NSG-Tg(hu-mSCF), 0 cGy (N = 10) . | NSG, 0 cGy (N = 8) . | NSG-Tg(hu-mSCF), 100 cGy (N = 7) . | NSG, 100 cGy (N = 10) . |
|---|---|---|---|---|
| Total human leukocytes: CD45+, % | 12.5 ± 6.5 | 18.3 ± 13 | 11.7 ± 14 | 11.4 ± 10.4 |
| B-cell lineage, % of CD45 | ||||
| CD20+ | 40.4 ± 17 | 58.0 ± 25 | 29.5 ± 16 | 45.7 ± 16.3 |
| CD20+/CD10+ | 5.4 ± 6.0 | 3.9 ± 1.8 | 14.4 ± 20 | 5.43 ± 4.1 |
| T-cell lineage, % of CD45 | ||||
| CD3+/CD4+ | 23.5 ± 9.9 | 27.5 ± 14 | 23.6 ± 16 | 21.6 ± 12 |
| RA:RO ratio | 0.4 ± 0.5 | 0.31 ± 0.30 | 0.6 ± 0.8 | 0.70 ± 0.51 |
| CD3+/CD8+ | 9.6 ± 6.7 | 15.5 ± 9.8 | 13.3 ± 10 | 10.2 ± 5.9 |
| RA:RO ratio | 2.6 ± 4.0 | 2.63 ± 4.5 | 2.64 ± 4.5 | 4.00 ± 3.8 |
| Myeloid/granulocytic, % of CD45 | ||||
| CD33+ | 1.2 ± 1.6 | 1.75 ± 1.2 | 0.8 ± 1.4 | 0.84 ± 1.3 |
| CD33+/64+ | 0.9 ± 1.1 | 0.01 ± 0.02 | 0.0 ± 0.0 | 0.43 ± 0.70 |
| CD14+ | 2.8 ± 2.4 | 1.45 ± 1.1 | 0.3 ± 0.2 | 1.85 ± 2.0 |
| Dendritic cell subsets, % of CD45 | ||||
| CD11c+ | 0.1 ± 0.1 | 0.39 ± 0.29 | 0.1 ± 0.1 | 0.12 ± 0.11 |
| CD123+ | 0.2 ± 0.2 | 0.37 ± 0.23 | 0.2 ± 0.2 | 0.23 ± 0.20 |
| Human cell subset . | NSG-Tg(hu-mSCF), 0 cGy (N = 10) . | NSG, 0 cGy (N = 8) . | NSG-Tg(hu-mSCF), 100 cGy (N = 7) . | NSG, 100 cGy (N = 10) . |
|---|---|---|---|---|
| Total human leukocytes: CD45+, % | 12.5 ± 6.5 | 18.3 ± 13 | 11.7 ± 14 | 11.4 ± 10.4 |
| B-cell lineage, % of CD45 | ||||
| CD20+ | 40.4 ± 17 | 58.0 ± 25 | 29.5 ± 16 | 45.7 ± 16.3 |
| CD20+/CD10+ | 5.4 ± 6.0 | 3.9 ± 1.8 | 14.4 ± 20 | 5.43 ± 4.1 |
| T-cell lineage, % of CD45 | ||||
| CD3+/CD4+ | 23.5 ± 9.9 | 27.5 ± 14 | 23.6 ± 16 | 21.6 ± 12 |
| RA:RO ratio | 0.4 ± 0.5 | 0.31 ± 0.30 | 0.6 ± 0.8 | 0.70 ± 0.51 |
| CD3+/CD8+ | 9.6 ± 6.7 | 15.5 ± 9.8 | 13.3 ± 10 | 10.2 ± 5.9 |
| RA:RO ratio | 2.6 ± 4.0 | 2.63 ± 4.5 | 2.64 ± 4.5 | 4.00 ± 3.8 |
| Myeloid/granulocytic, % of CD45 | ||||
| CD33+ | 1.2 ± 1.6 | 1.75 ± 1.2 | 0.8 ± 1.4 | 0.84 ± 1.3 |
| CD33+/64+ | 0.9 ± 1.1 | 0.01 ± 0.02 | 0.0 ± 0.0 | 0.43 ± 0.70 |
| CD14+ | 2.8 ± 2.4 | 1.45 ± 1.1 | 0.3 ± 0.2 | 1.85 ± 2.0 |
| Dendritic cell subsets, % of CD45 | ||||
| CD11c+ | 0.1 ± 0.1 | 0.39 ± 0.29 | 0.1 ± 0.1 | 0.12 ± 0.11 |
| CD123+ | 0.2 ± 0.2 | 0.37 ± 0.23 | 0.2 ± 0.2 | 0.23 ± 0.20 |
Nonirradiated NSG and NSG-Tg(hu-mSCF) newborn mice, and 100-cGy irradiated NSG and NSG-Tg(hu-mSCF) newborn mice were engrafted with human HSCs as described in “Engraftment of mice human HSCs.” Mice were analyzed by flow cytometry for the percentages of human CD45+ cells and human CD45+ cells that were positive for specific lineage markers in the blood at 12 weeks of age. Data are mean ± SD. No significant differences between groups were observed.