Biological functions of RUNX1 mutants assayed in this study
| RUNX1 gene . | Subcellular localization . | DNA-binding ability . | CBFβ-binding ability . | Transactivation activity . | Abilities of homodimerization . | Interference of RUNX1 oligomer formation . |
|---|---|---|---|---|---|---|
| WT | N | → | → | → | + | − |
| H78Q | N | ↓ | → | ↓ | + | − |
| V91fs-ter94 | N and C | ↓ | ↓ | ↓ | − | + |
| R139G | N and C | ↓ | → | ↓ | + | − |
| D171G | N | ↓ | → | ↓ | + | + |
| R174Q | N | ↓ | → | ↓ | + | − |
| R293X | N | → | ↑ | ↓ | − | − |
| G381fs-ter570 | N | → | → | → | − | + |
| RUNX1 gene . | Subcellular localization . | DNA-binding ability . | CBFβ-binding ability . | Transactivation activity . | Abilities of homodimerization . | Interference of RUNX1 oligomer formation . |
|---|---|---|---|---|---|---|
| WT | N | → | → | → | + | − |
| H78Q | N | ↓ | → | ↓ | + | − |
| V91fs-ter94 | N and C | ↓ | ↓ | ↓ | − | + |
| R139G | N and C | ↓ | → | ↓ | + | − |
| D171G | N | ↓ | → | ↓ | + | + |
| R174Q | N | ↓ | → | ↓ | + | − |
| R293X | N | → | ↑ | ↓ | − | − |
| G381fs-ter570 | N | → | → | → | − | + |
N indicates nuclear localization; C, cytoplasmic localization; →, not changed; ↓, decreased; ↑, increased; +, interfered; and −, not interfered.