Enzyme kinetic parameters
| Enzyme/cofactor . | Substrate . | Km, nM . | kcat, s−1 . | kcat/Km, M−1s−1 . |
|---|---|---|---|---|
| FXIa* | ||||
| N9 | 45 ± 2 | 0.81 ± 0.01 | (18 ± 1) × 106 | |
| N9-GP | 46 ± 6 | 0.76 ± 0.03 | (17 ± 2) × 106 | |
| FVIIa† | ||||
| TF/PS:PC | N9 | 74 ± 3 | 0.50 ± 0.01 | (6.7 ± 0.2) × 106 |
| TF/PS:PC | N9-GP | 122 ± 10 | 0.38 ± 0.02 | (3.1 ± 0.1) × 106 |
| N9a‡ | ||||
| FVIIIa/PS:PS | FX | 11.4 ± 0.3 | 8.7 ± 0.8 | (7.6 ± 0.5) × 108 |
| [N9-GP]a‡ | ||||
| FVIIIa/PS:PC | FX | 12.9 ± 0.9 | 9.2 ± 0.1 | (7.1 ± 0.4) × 108 |
| Enzyme/cofactor . | Substrate . | Km, nM . | kcat, s−1 . | kcat/Km, M−1s−1 . |
|---|---|---|---|---|
| FXIa* | ||||
| N9 | 45 ± 2 | 0.81 ± 0.01 | (18 ± 1) × 106 | |
| N9-GP | 46 ± 6 | 0.76 ± 0.03 | (17 ± 2) × 106 | |
| FVIIa† | ||||
| TF/PS:PC | N9 | 74 ± 3 | 0.50 ± 0.01 | (6.7 ± 0.2) × 106 |
| TF/PS:PC | N9-GP | 122 ± 10 | 0.38 ± 0.02 | (3.1 ± 0.1) × 106 |
| N9a‡ | ||||
| FVIIIa/PS:PS | FX | 11.4 ± 0.3 | 8.7 ± 0.8 | (7.6 ± 0.5) × 108 |
| [N9-GP]a‡ | ||||
| FVIIIa/PS:PC | FX | 12.9 ± 0.9 | 9.2 ± 0.1 | (7.1 ± 0.4) × 108 |
Michaelis-Menten parameters were measured in the presence of relevant cofactors as indicated. N9a and [N9-GP]a denote activated N9 and N9-GP, respectively.
FX indicates factor X; FV, factor V; TF, tissue factor; PS, phosphatidylserine; and PC, phosphatidylcholine.
See Figure 2A.
See Figure 2B.
See Figure 2D.