Clonotypic analysis of KK10-specific CD8+ T-cell populations harboring public TRBV4-3/TRBJ1-3 sequences
| Patient/TRBV . | CDR3 sequence, aa . | TRBJ . | Frequency, % . |
|---|---|---|---|
| 02.011, CD4c = 1227, pVL = 1880 | |||
| 4-3*† | CASSPGQLGNTIY*† | 1-3*† | 54 |
| 7-6 | CASRLLGGGQETQY | 2-5 | 35 |
| 4-3 | CASSPGVFGVTQY | 2-3 | 11 |
| 01.01 DOF, CD4c = 716, pVL = 4400 | |||
| 4-3 | CASSQGQYGNEQF | 2-1 | 37 |
| 20-1 | CSARREANYGYT | 1-2 | 22 |
| 4-3 | CASSEGANAYEQY | 2-7 | 9 |
| 3-1 | CASSQDGVYSNQPQH | 1-5 | 6 |
| 4-3 | CASSMGQNSNEQY | 2-7 | 4 |
| 4-3 | CASSQGLSSNEQF | 2-1 | 3 |
| 18 | CASSLGGLDIEQY | 2-7 | 3 |
| 27 | CATSGVTGELF | 2-2 | 3 |
| 4-3*† | CASSPGQLGNTIY*† | 1-3*† | 1 |
| 4-3* | CASSPGVLGNTIY* | 1-3* | 1 |
| 20-1 | CARREANYGYT | 1-2 | 1 |
| 27 | CASAMTGEGYGYT | 1-2 | 1 |
| 27 | CASSGGAHTEAF | 1-1 | 1 |
| 27 | CASSSKTGELF | 2-2 | 1 |
| 27 | CASSSRTGELF | 2-2 | 1 |
| 7-2 | CASSSTRGTEAF | 1-1 | 1 |
| L8146, CD4c = 332, pVL = 202590 | |||
| 4-3*‡ | CASSQGQLGNTIY*‡ | 1-3*‡ | 40 |
| 7-6 | CASSLGGTNHGYT | 1-2 | 13 |
| 7-2 | CASSLVLAIEQY | 2-7 | 7 |
| 9 | CASSPGTGKNIQY | 2-4 | 7 |
| 4-1 | CASSQEGVNTEAF | 1-1 | 4 |
| 4-3 | CASSQAQGLSNSPLH | 1-6 | 4 |
| 5-1 | CASSRTQGPNTDTQY | 2-3 | 4 |
| 7-6 | CASSLDHLAGVNNEQF | 2-1 | 4 |
| 6-1 | CASSGQLLEAF | 1-1 | 2 |
| 7-8 | CASSLEGSRNTEAF | 1-1 | 2 |
| 11-2 | CASSQPPDRGYGYT | 1-2 | 2 |
| 12-4 | CASSLVGSYNEQF | 2-1 | 2 |
| 12-4 | CASSTTSGRYEQY | 2-7 | 2 |
| 18 | CASSFGQGAIEQY | 2-7 | 2 |
| 27 | CASSLSSDEHGYT | 1-2 | 2 |
| 11.007, CD4c = 716, pVL = 4400 | |||
| 20-1 | CSARDLGLAGDTDTQY | 2-3 | 84 |
| 7-9 | CASSLDSYEQY | 2-7 | 13 |
| 4-3*‡ | CASSQGQLGNTIY*‡ | 1-3*‡ | 2 |
| 28 | CASSLGIPGTAQWVYGYT | 1-2 | 2 |
| LTS 12, CD4c = 462, pVL = 17254 | |||
| 28 | CASSLRGGNTDTQY | 2-3 | 61 |
| 24-1 | CATSETGELF | 2-2 | 29 |
| 4-3*§ | CASSPGQFGNTIY*§ | 1-3*§ | 9 |
| 30 | CAWSLTGMNQPQH | 1-5 | 1 |
| LTS 57, CD4c = 1050, pVL = 1900 | |||
| 27 | CASMGGANTEAF | 1-1 | 88 |
| 27 | CASSPTTYGYT | 1-2 | 3 |
| 4-3*§ | CASSPGQFGNTIY*§ | 1-3*§ | 3 |
| 10-3 | CAISEYGNAASPLH | 1-6 | 2 |
| 9 | CASSVLGTSGGAEQF | 2-1 | 2 |
| 20-1 | CSARDWASGLSSYEQY | 2-7 | 2 |
| Patient/TRBV . | CDR3 sequence, aa . | TRBJ . | Frequency, % . |
|---|---|---|---|
| 02.011, CD4c = 1227, pVL = 1880 | |||
| 4-3*† | CASSPGQLGNTIY*† | 1-3*† | 54 |
| 7-6 | CASRLLGGGQETQY | 2-5 | 35 |
| 4-3 | CASSPGVFGVTQY | 2-3 | 11 |
| 01.01 DOF, CD4c = 716, pVL = 4400 | |||
| 4-3 | CASSQGQYGNEQF | 2-1 | 37 |
| 20-1 | CSARREANYGYT | 1-2 | 22 |
| 4-3 | CASSEGANAYEQY | 2-7 | 9 |
| 3-1 | CASSQDGVYSNQPQH | 1-5 | 6 |
| 4-3 | CASSMGQNSNEQY | 2-7 | 4 |
| 4-3 | CASSQGLSSNEQF | 2-1 | 3 |
| 18 | CASSLGGLDIEQY | 2-7 | 3 |
| 27 | CATSGVTGELF | 2-2 | 3 |
| 4-3*† | CASSPGQLGNTIY*† | 1-3*† | 1 |
| 4-3* | CASSPGVLGNTIY* | 1-3* | 1 |
| 20-1 | CARREANYGYT | 1-2 | 1 |
| 27 | CASAMTGEGYGYT | 1-2 | 1 |
| 27 | CASSGGAHTEAF | 1-1 | 1 |
| 27 | CASSSKTGELF | 2-2 | 1 |
| 27 | CASSSRTGELF | 2-2 | 1 |
| 7-2 | CASSSTRGTEAF | 1-1 | 1 |
| L8146, CD4c = 332, pVL = 202590 | |||
| 4-3*‡ | CASSQGQLGNTIY*‡ | 1-3*‡ | 40 |
| 7-6 | CASSLGGTNHGYT | 1-2 | 13 |
| 7-2 | CASSLVLAIEQY | 2-7 | 7 |
| 9 | CASSPGTGKNIQY | 2-4 | 7 |
| 4-1 | CASSQEGVNTEAF | 1-1 | 4 |
| 4-3 | CASSQAQGLSNSPLH | 1-6 | 4 |
| 5-1 | CASSRTQGPNTDTQY | 2-3 | 4 |
| 7-6 | CASSLDHLAGVNNEQF | 2-1 | 4 |
| 6-1 | CASSGQLLEAF | 1-1 | 2 |
| 7-8 | CASSLEGSRNTEAF | 1-1 | 2 |
| 11-2 | CASSQPPDRGYGYT | 1-2 | 2 |
| 12-4 | CASSLVGSYNEQF | 2-1 | 2 |
| 12-4 | CASSTTSGRYEQY | 2-7 | 2 |
| 18 | CASSFGQGAIEQY | 2-7 | 2 |
| 27 | CASSLSSDEHGYT | 1-2 | 2 |
| 11.007, CD4c = 716, pVL = 4400 | |||
| 20-1 | CSARDLGLAGDTDTQY | 2-3 | 84 |
| 7-9 | CASSLDSYEQY | 2-7 | 13 |
| 4-3*‡ | CASSQGQLGNTIY*‡ | 1-3*‡ | 2 |
| 28 | CASSLGIPGTAQWVYGYT | 1-2 | 2 |
| LTS 12, CD4c = 462, pVL = 17254 | |||
| 28 | CASSLRGGNTDTQY | 2-3 | 61 |
| 24-1 | CATSETGELF | 2-2 | 29 |
| 4-3*§ | CASSPGQFGNTIY*§ | 1-3*§ | 9 |
| 30 | CAWSLTGMNQPQH | 1-5 | 1 |
| LTS 57, CD4c = 1050, pVL = 1900 | |||
| 27 | CASMGGANTEAF | 1-1 | 88 |
| 27 | CASSPTTYGYT | 1-2 | 3 |
| 4-3*§ | CASSPGQFGNTIY*§ | 1-3*§ | 3 |
| 10-3 | CAISEYGNAASPLH | 1-6 | 2 |
| 9 | CASSVLGTSGGAEQF | 2-1 | 2 |
| 20-1 | CSARDWASGLSSYEQY | 2-7 | 2 |
Data are shown for 6 of 19 KK10-specific CD8+ T-cell populations sort-purified directly ex vivo from HLA-B*2705+ patients infected with HIV-1. CD4+ T-cell counts (CD4c) and plasma viral load (pVL) are indicated at time of sample.
Bold values represent all TRBV4-3/TRBJ1-3 clonotypes.
First of 3 public TRBV4-3/TRBJ1-3 clonotypes.
Second of 3 public TRBV4-3/TRBJ1-3 clonotypes.
Third of 3 public TRBV4-3/TRBJ1-3 clonotypes.