Bone marrow cell counts
| Bone marrow . | Control . | KO . |
|---|---|---|
| Total cell count | 72.0 + 15.0 | 71.0 + 5.5 |
| Leukocyte cell count | 20.8 + 3.3 | 6.5 + 4.9* |
| B220+ B lymphocytes (%) | 16.4 + 5.1 | 44.2 + 8.6* |
| B220+ B-lymphocyte count | 3.4 + 0.5 | 2.9 + 2.8 |
| B220+IgM− lymphocytes (%) | 82.3 + 17.8 | 51.7 + 18.3 |
| B220+IgM− lymphocyte count | 2.8 + 0.4 | 1.5 + 1.4 |
| pro-B (B220+CD43+IgM−) lymphocytes (%) | 4.6 + 0.7 | 2.4 + 1.1* |
| pro-B (B220+CD43+IgM−) lymphocyte count | 0.13 + 0.02 | 0.04 + 0.02* |
| Myeloid (CD11b+) cells (%) | 54.9 + 4.1 | 22.8 + 7.3* |
| Myeloid (CD11b+) count | 11.4 + 1.8 | 1.5 + 1.1* |
| Granulocytes (CD11bhi Gr1hi; %) | 35.2 + 5.0 | 1.3 + 0.9* |
| Granulocytes (CD11bhi Gr1hi) count | 7.3 + 1.2 | 0.1 + 0.1* |
| Immature myeloid (CD11bhi Gr1lo; %) | 18.1 + 4.6 | 21.7 + 12.9 |
| Immature myeloid (CD11bhi Gr1lo) count | 6.0 + 1.0 | 1.4 + 0.9* |
| CD11b− Gr1lo (%) | 3.7 + 1.3 | 13.6 + 3.1* |
| CD11b− Gr1lo count | 0.8 + 0.3 | 0.9 + 0.2 |
| Bone marrow . | Control . | KO . |
|---|---|---|
| Total cell count | 72.0 + 15.0 | 71.0 + 5.5 |
| Leukocyte cell count | 20.8 + 3.3 | 6.5 + 4.9* |
| B220+ B lymphocytes (%) | 16.4 + 5.1 | 44.2 + 8.6* |
| B220+ B-lymphocyte count | 3.4 + 0.5 | 2.9 + 2.8 |
| B220+IgM− lymphocytes (%) | 82.3 + 17.8 | 51.7 + 18.3 |
| B220+IgM− lymphocyte count | 2.8 + 0.4 | 1.5 + 1.4 |
| pro-B (B220+CD43+IgM−) lymphocytes (%) | 4.6 + 0.7 | 2.4 + 1.1* |
| pro-B (B220+CD43+IgM−) lymphocyte count | 0.13 + 0.02 | 0.04 + 0.02* |
| Myeloid (CD11b+) cells (%) | 54.9 + 4.1 | 22.8 + 7.3* |
| Myeloid (CD11b+) count | 11.4 + 1.8 | 1.5 + 1.1* |
| Granulocytes (CD11bhi Gr1hi; %) | 35.2 + 5.0 | 1.3 + 0.9* |
| Granulocytes (CD11bhi Gr1hi) count | 7.3 + 1.2 | 0.1 + 0.1* |
| Immature myeloid (CD11bhi Gr1lo; %) | 18.1 + 4.6 | 21.7 + 12.9 |
| Immature myeloid (CD11bhi Gr1lo) count | 6.0 + 1.0 | 1.4 + 0.9* |
| CD11b− Gr1lo (%) | 3.7 + 1.3 | 13.6 + 3.1* |
| CD11b− Gr1lo count | 0.8 + 0.3 | 0.9 + 0.2 |
Cell counts of total bone marrow cells and leukocyte subsets (determined after lysis of red blood cells; × 106/animal), percentages of the indicated immunophenotypic subsets, and calculated numbers of leukocyte subsets (× 106/animal) in at least 3 different control and KO mice.
P < .05 by Student t test.