Table 2 Stereotypic HCDR3 rearrangements... | American Society of Hematology

Table 2

Stereotypic HCDR3 rearrangements of lymphoproliferations from CDR, MDR, and miR-15a/16-1–deleted and TCL1-transgenic²⁶ mice

Animal	V_H	V_H	N	D_H	N	J_H	J_H	CDR3 length	Stereotype set
MDR^+/− #175-PB	V261	AG	DR	YGY		WYFDV	1	12	I
TCL1_001	V261	AG		DRRGY		WYFDV	1	12	I
TCL1_002	V261	AG		DRTGY		WYFDV	1	12	I
CDR^fl/flCD19-Cre #178	V126	AR	DP	YYYGSS	L	YYFDY	2	16	II
MDR^fl/+CD19-Cre #211	V126	AR		YYYGSSY		YFDY	2	13	II
TCL1_003	V4S1	AR		HYYGSSY		FDY	2	12	II
TCL1_004	V4S1	AR		HYYGSSY		FDV	1	12	II
TCL1_007	V332	AR		IYYYGSSY		AMDY	4	14	III
TCL1_008	V1S61	AR		SYYDGSYY		AMDY	4	14	III
CDR^+/+ #114	V235	MRY		GNY		WYFDV	1	11	IV
CDR^+/− #181	V235	MRY		GNY		WYFDV	1	11	IV
MDR^−/− #138	V153	MR		YGNY		WYFDV	1	11	IV
CDR^+/−CD19-Cre #225	V235	MRY		SNY		WYFDV	1	11	IV
MDR^+/− #27	V235	MR		YSNY		WYFDV	1	11	IV
TCL1_006	V153	MR		YSNY		WYFDV	1	11	IV
CDR^+/− #67	V332	AR	R	DYGSSY		WYFDV	1	14	V
CDR^fl/flCD19-Cre #202	V332	AR	I	YYGSSY		WYFDV	1	14	V
TCL1_010	V332	AR	I	YYGSSY		WYFDV	1	14	V
MDR^fl/−CD19-Cre #219	V128			YYGSSY		WYFDV	1	11	V
TCL1_005	V153	MR		YGSSY		WYFDV	1	12	V
TCL1_009	V332	AR	R	YYGSS		WYFDV	1	13	V
miR^fl/−CD19-Cre #10	V328	A		IYYGNY		WYFDV	1	12	prov. VI
CDR^fl/−CD19-Cre #184-J1	V163	AS		YYGY		WYFDV	1	11	prov. VI
MDR^fl/−CD19Cre #99	V328	AR		YYSNY		WYFDV	1	12	prov. VI
miR^fl/−CD19Cre #234	V328	AR	GEK	YSNY		WYFDV	1	14	prov. VI
CDR^fl/+CD19-Cre #47	V186	AR		IYDGYY		YFDY	2	12	prov. VII
CDR^fl/−CD19-Cre #224	V122	ARD		DGYY		YFDY	2	11	prov. VII
MDR^fl/−CD19-Cre #197	V227	A	SP	NWD		WYFDV	1	11	prov. VIII
MDR^+/+CD19-Cre #212	V186	AR		NWD		WYFDV	1	10	prov. VIII

Animal	V_H	V_H	N	D_H	N	J_H	J_H	CDR3 length	Stereotype set
MDR^+/− #175-PB	V261	AG	DR	YGY		WYFDV	1	12	I
TCL1_001	V261	AG		DRRGY		WYFDV	1	12	I
TCL1_002	V261	AG		DRTGY		WYFDV	1	12	I
CDR^fl/flCD19-Cre #178	V126	AR	DP	YYYGSS	L	YYFDY	2	16	II
MDR^fl/+CD19-Cre #211	V126	AR		YYYGSSY		YFDY	2	13	II
TCL1_003	V4S1	AR		HYYGSSY		FDY	2	12	II
TCL1_004	V4S1	AR		HYYGSSY		FDV	1	12	II
TCL1_007	V332	AR		IYYYGSSY		AMDY	4	14	III
TCL1_008	V1S61	AR		SYYDGSYY		AMDY	4	14	III
CDR^+/+ #114	V235	MRY		GNY		WYFDV	1	11	IV
CDR^+/− #181	V235	MRY		GNY		WYFDV	1	11	IV
MDR^−/− #138	V153	MR		YGNY		WYFDV	1	11	IV
CDR^+/−CD19-Cre #225	V235	MRY		SNY		WYFDV	1	11	IV
MDR^+/− #27	V235	MR		YSNY		WYFDV	1	11	IV
TCL1_006	V153	MR		YSNY		WYFDV	1	11	IV
CDR^+/− #67	V332	AR	R	DYGSSY		WYFDV	1	14	V
CDR^fl/flCD19-Cre #202	V332	AR	I	YYGSSY		WYFDV	1	14	V
TCL1_010	V332	AR	I	YYGSSY		WYFDV	1	14	V
MDR^fl/−CD19-Cre #219	V128			YYGSSY		WYFDV	1	11	V
TCL1_005	V153	MR		YGSSY		WYFDV	1	12	V
TCL1_009	V332	AR	R	YYGSS		WYFDV	1	13	V
miR^fl/−CD19-Cre #10	V328	A		IYYGNY		WYFDV	1	12	prov. VI
CDR^fl/−CD19-Cre #184-J1	V163	AS		YYGY		WYFDV	1	11	prov. VI
MDR^fl/−CD19Cre #99	V328	AR		YYSNY		WYFDV	1	12	prov. VI
miR^fl/−CD19Cre #234	V328	AR	GEK	YSNY		WYFDV	1	14	prov. VI
CDR^fl/+CD19-Cre #47	V186	AR		IYDGYY		YFDY	2	12	prov. VII
CDR^fl/−CD19-Cre #224	V122	ARD		DGYY		YFDY	2	11	prov. VII
MDR^fl/−CD19-Cre #197	V227	A	SP	NWD		WYFDV	1	11	prov. VIII
MDR^+/+CD19-Cre #212	V186	AR		NWD		WYFDV	1	10	prov. VIII

Order of cases is according to the alignment obtained after hierarchical clustering of all HCDR3 sequences. Cases that showed ≥ 80% amino acid sequence homology in the CDR3 to the next neighbor/s are grouped. MDR and miR cases are derived from Klein et al,²² TCL1 cases from Yan et al.²⁶ Stereotype sets defined by Yan et al (sets I-V²⁶ and provisional [prov.] sets VI-VIII identified in the present work) are indicated in the right column.

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