Serial dilution of 4 VHR-ALL xenografts by intrafemoral and intravenous transplantation
| Patient . | Round of transplantation . | Cell dilution . | Intrafemoral engrafted/total . | Intravenous engrafted/total . | Mean engraftment in bone marrow, % . |
|---|---|---|---|---|---|
| VHR-01 | Second | 1 000 000 | 2/4 | 3/3 | 88 |
| 10 000 | 3/3 | 3/3* | 86 | ||
| 100* | 2/3* | 0/3 | 96 | ||
| VHR-02 | Third | 1 000 000 | 1/2 | 2/3 | 94 |
| 10 000 | 3/3 | 1/3* | 97 | ||
| 100* | 2/3* | 0/3 | 90 | ||
| VHR-03 | Third | 1 000 000 | 2/2 | 2/2 | 94 |
| 10 000 | 2/2 | 2/2* | 97 | ||
| 100* | 1/2* | 0/2 | 77 | ||
| VHR-04 | Second | 1 000 000 | 2/2 | 2/3 | 99 |
| 10 000 | 1/3 | 2/3* | 99 | ||
| 100* | 1/3* | 0/3 | 99 |
| Patient . | Round of transplantation . | Cell dilution . | Intrafemoral engrafted/total . | Intravenous engrafted/total . | Mean engraftment in bone marrow, % . |
|---|---|---|---|---|---|
| VHR-01 | Second | 1 000 000 | 2/4 | 3/3 | 88 |
| 10 000 | 3/3 | 3/3* | 86 | ||
| 100* | 2/3* | 0/3 | 96 | ||
| VHR-02 | Third | 1 000 000 | 1/2 | 2/3 | 94 |
| 10 000 | 3/3 | 1/3* | 97 | ||
| 100* | 2/3* | 0/3 | 90 | ||
| VHR-03 | Third | 1 000 000 | 2/2 | 2/2 | 94 |
| 10 000 | 2/2 | 2/2* | 97 | ||
| 100* | 1/2* | 0/2 | 77 | ||
| VHR-04 | Second | 1 000 000 | 2/2 | 2/3 | 99 |
| 10 000 | 1/3 | 2/3* | 99 | ||
| 100* | 1/3* | 0/3 | 99 |
Four VHR-ALL patients from different amplification rounds were transplanted intrafemorally and intravenously. Engraftment was considered positive when 1% of human CD19/CD45+ mouse CD45− cells were detected in peripheral blood by flow cytometry, and spleens and bone marrows were engrafted at harvest.
Lowest dilution that resulted in engraftment.