Systematic summary of surface markers that uniquely separates the 3 monocyte subsets
| Marker . | MFI minus isotype MFI (mean ± SD) . | Average fold change . | P . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Classical . | Intermediate . | Nonclassical . | C vs I . | C vs N . | I vs N . | C vs I . | C vs N . | I vs N . | |
| Classical monocyte subset | |||||||||
| CD62L (n = 5) | 2715 ± 1555 | 790 ± 527 | 605 ± 426 | 3.4 | 4.5 | 1.3 | * | * | NS |
| CD54 (n = 6) | 2385 + 588 | 5270 ± 1207 | 4326 ± 1717 | −2.2 | −1.8 | 1.2 | † | * | NS |
| CD40 (n = 6) | 20 ± 24 | 126 ± 40 | 90 ± 45 | −6.4 | −4.5 | 1.4 | ‡ | * | NS |
| CD36 (n = 5) | 33 184 ± 13 648 | 15 853 ± 4591 | 3244 ± 950 | 2.1 | 10.2 | 4.9 | * | ‡ | NS |
| CD99 (n = 5) | 22 692 ± 6961 | 8914 ± 3415 | 1594 ± 1074 | 2.5 | 14.2 | 5.6 | † | ‡ | NS |
| CCR1 (n = 6) | 905 ± 440 | 355 ± 188 | 55 ± 63 | 2.6 | 16.4 | 6.4 | † | ‡ | NS |
| P2XR1 (n = 5) | 182 ± 82 | 847 ± 202 | 1026 ± 378 | −4.7 | −5.6 | −1.2 | † | ‡ | NS |
| CCR2 (n = 6) | 968 ± 440 | 36 ± 59 | 4 ± 7 | 26.7 | 230.5 | 8.6 | ‡ | ‡ | NS |
| CLEC4D (n = 7) | 1260 ± 778 | 115 ± 110 | 6 ± 15 | 11.0 | 200.1 | 18.2 | ‡ | ‡ | NS |
| CLEC5A (n = 5) | 753 ± 209 | 217 ± 34 | 43 ± 23 | 3.5 | 17.4 | 5.0 | ‡ | ‡ | NS |
| CXCR1 (n = 6) | 159 ± 58 | 32 ± 18 | 7 ± 8 | 5.0 | 21.5 | 4.3 | ‡ | ‡ | NS |
| CXCR2 (n = 6) | 557 ± 239 | 78 ± 42 | 23 ± 28 | 7.1 | 24.6 | 3.4 | ‡ | ‡ | NS |
| IL13Ra1 (n = 8) | 971 ± 647 | 107 ± 151 | 39 ± 92 | 9.1 | 24.6 | 2.7 | ‡ | ‡ | NS |
| CD64 (n = 4) | 2135 ± 449 | 931 ± 193 | 236 ± 58 | 2.3 | 9.0 | 3.9 | ‡ | ‡ | * |
| Intermediate monocyte subset | |||||||||
| HLA-ABC (n = 6) | 29 026 + 5768 | 36 894 + 5088 | 27 796 + 3563 | −1.3 | 1.0 | 1.3 | * | NS | * |
| CLEC10A (n = 6) | 9 ± 14 | 64 ± 40 | 15 ± 12 | −6.9 | −1.6 | 4.3 | † | NS | * |
| GFRA2 (n = 7) | 6 ± 10 | 138 ± 95 | 37 ± 25 | −6.2 | −23.0 | 3.7 | † | NS | * |
| HLA-DR (n = 5) | 3916 ± 1363 | 31 755 ± 9410 | 12 584 ± 5924 | −8.1 | −3.2 | 2.5 | ‡ | NS | † |
| Nonclassical monocyte subset | |||||||||
| CD163 (n = 5) | 4310 ± 2587 | 4678 ± 2295 | 632 ± 526 | −1.1 | 6.8 | 7.4 | NS | * | * |
| CD115 (n = 4) | 295 ± 170 | 470 ± 199 | 865 ± 187 | −1.6 | −2.9 | −1.8 | NS | † | * |
| CD11b (n = 4) | 4043 ± 1197 | 3617 ± 1365 | 915 ± 684 | 1.1 | 4.4 | 4.0 | NS | † | * |
| SLAN (n = 5) | 32.4 ± 21.0 | 16.8 ± 17.5 | 119.0 ± 56.9 | 1.9 | −3.7 | −7.1 | NS | † | † |
| CD1d (n = 4) | 1072 ± 528 | 810 ± 297 | 193 ± 138 | 1.3 | 5.5 | 4.2 | NS | ‡ | † |
| CCR5 (n = 7) | 219 ± 62 | 249 ± 116 | 30 ± 47 | −1.1 | 7.3 | 8.3 | NS | ‡ | ‡ |
| CD294 (n = 9) | 131 ± 124 | 207 ± 124 | 476 ± 147 | −1.6 | −3.6 | −2.3 | NS | ‡ | ‡ |
| Siglec10 (n = 9) | 76 ± 92 | 584 ± 340 | 1615 ± 659 | −7.7 | −21.3 | −2.8 | * | ‡ | ‡ |
| Marker . | MFI minus isotype MFI (mean ± SD) . | Average fold change . | P . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Classical . | Intermediate . | Nonclassical . | C vs I . | C vs N . | I vs N . | C vs I . | C vs N . | I vs N . | |
| Classical monocyte subset | |||||||||
| CD62L (n = 5) | 2715 ± 1555 | 790 ± 527 | 605 ± 426 | 3.4 | 4.5 | 1.3 | * | * | NS |
| CD54 (n = 6) | 2385 + 588 | 5270 ± 1207 | 4326 ± 1717 | −2.2 | −1.8 | 1.2 | † | * | NS |
| CD40 (n = 6) | 20 ± 24 | 126 ± 40 | 90 ± 45 | −6.4 | −4.5 | 1.4 | ‡ | * | NS |
| CD36 (n = 5) | 33 184 ± 13 648 | 15 853 ± 4591 | 3244 ± 950 | 2.1 | 10.2 | 4.9 | * | ‡ | NS |
| CD99 (n = 5) | 22 692 ± 6961 | 8914 ± 3415 | 1594 ± 1074 | 2.5 | 14.2 | 5.6 | † | ‡ | NS |
| CCR1 (n = 6) | 905 ± 440 | 355 ± 188 | 55 ± 63 | 2.6 | 16.4 | 6.4 | † | ‡ | NS |
| P2XR1 (n = 5) | 182 ± 82 | 847 ± 202 | 1026 ± 378 | −4.7 | −5.6 | −1.2 | † | ‡ | NS |
| CCR2 (n = 6) | 968 ± 440 | 36 ± 59 | 4 ± 7 | 26.7 | 230.5 | 8.6 | ‡ | ‡ | NS |
| CLEC4D (n = 7) | 1260 ± 778 | 115 ± 110 | 6 ± 15 | 11.0 | 200.1 | 18.2 | ‡ | ‡ | NS |
| CLEC5A (n = 5) | 753 ± 209 | 217 ± 34 | 43 ± 23 | 3.5 | 17.4 | 5.0 | ‡ | ‡ | NS |
| CXCR1 (n = 6) | 159 ± 58 | 32 ± 18 | 7 ± 8 | 5.0 | 21.5 | 4.3 | ‡ | ‡ | NS |
| CXCR2 (n = 6) | 557 ± 239 | 78 ± 42 | 23 ± 28 | 7.1 | 24.6 | 3.4 | ‡ | ‡ | NS |
| IL13Ra1 (n = 8) | 971 ± 647 | 107 ± 151 | 39 ± 92 | 9.1 | 24.6 | 2.7 | ‡ | ‡ | NS |
| CD64 (n = 4) | 2135 ± 449 | 931 ± 193 | 236 ± 58 | 2.3 | 9.0 | 3.9 | ‡ | ‡ | * |
| Intermediate monocyte subset | |||||||||
| HLA-ABC (n = 6) | 29 026 + 5768 | 36 894 + 5088 | 27 796 + 3563 | −1.3 | 1.0 | 1.3 | * | NS | * |
| CLEC10A (n = 6) | 9 ± 14 | 64 ± 40 | 15 ± 12 | −6.9 | −1.6 | 4.3 | † | NS | * |
| GFRA2 (n = 7) | 6 ± 10 | 138 ± 95 | 37 ± 25 | −6.2 | −23.0 | 3.7 | † | NS | * |
| HLA-DR (n = 5) | 3916 ± 1363 | 31 755 ± 9410 | 12 584 ± 5924 | −8.1 | −3.2 | 2.5 | ‡ | NS | † |
| Nonclassical monocyte subset | |||||||||
| CD163 (n = 5) | 4310 ± 2587 | 4678 ± 2295 | 632 ± 526 | −1.1 | 6.8 | 7.4 | NS | * | * |
| CD115 (n = 4) | 295 ± 170 | 470 ± 199 | 865 ± 187 | −1.6 | −2.9 | −1.8 | NS | † | * |
| CD11b (n = 4) | 4043 ± 1197 | 3617 ± 1365 | 915 ± 684 | 1.1 | 4.4 | 4.0 | NS | † | * |
| SLAN (n = 5) | 32.4 ± 21.0 | 16.8 ± 17.5 | 119.0 ± 56.9 | 1.9 | −3.7 | −7.1 | NS | † | † |
| CD1d (n = 4) | 1072 ± 528 | 810 ± 297 | 193 ± 138 | 1.3 | 5.5 | 4.2 | NS | ‡ | † |
| CCR5 (n = 7) | 219 ± 62 | 249 ± 116 | 30 ± 47 | −1.1 | 7.3 | 8.3 | NS | ‡ | ‡ |
| CD294 (n = 9) | 131 ± 124 | 207 ± 124 | 476 ± 147 | −1.6 | −3.6 | −2.3 | NS | ‡ | ‡ |
| Siglec10 (n = 9) | 76 ± 92 | 584 ± 340 | 1615 ± 659 | −7.7 | −21.3 | −2.8 | * | ‡ | ‡ |
C indicates classical monocyte subset; I, intermediate monocyte subset; N, nonclassical monocyte subset; n, the number of individuals tested for each surface marker; and NS, not significant.
P < .05 (ANOVA and post-hoc Tukey test).
P < .01 (ANOVA and post-hoc Tukey test).
P < .001 (ANOVA and post-hoc Tukey test).