Colony-forming potential of purified population of Erg+/Mld2 bone marrow cells
| Cell population/genotype . | No. of colony-forming cells per 100 cells plated* . | |||||||
|---|---|---|---|---|---|---|---|---|
| Blast . | Granulocyte . | Granulocyte-macrophage . | Macrophage . | Eosinophil . | Megakaryocyte . | |||
| Total . | Multicentric . | Dispersed . | ||||||
| CD34− | ||||||||
| Erg+/+ | 18.4 ± 6.0 | 11.6 ± 4.6 | 6.9 ± 3.0 | 0.1 ± 0.4 | 0 | 3.9 ± 3.8 | 0 | 5.1 ± 6.2 |
| Erg+/Mld2 | 6.0 ± 3.8† | 4.0 ± 3.3† | 2.0 ± 1.9† | 0 | 0.1 ± 0.4 | 2.6 ± 2.4 | 0 | 8.1 ± 7.1 |
| CD34+Flt3R− | ||||||||
| Erg+/+ | 31.3 ± 8.1 | 27.3 ± 9.5 | 4.0 ± 1.7 | 3.9 ± 3.9 | 4.4 ± 3.8 | 4.0 ± 3.6 | 0 | 5.8 ± 2.0 |
| Erg+/Mld2 | 13.7 ± 10.5† | 12.3 ± 9.9† | 1.4 ± 2.1† | 3.3 ± 2.9 | 1.1 ± 0.9 | 0.7 ± 1.0 | 0.4 ± 0.8 | 5.1 ± 4.1 |
| Cell population/genotype . | No. of colony-forming cells per 100 cells plated* . | |||||||
|---|---|---|---|---|---|---|---|---|
| Blast . | Granulocyte . | Granulocyte-macrophage . | Macrophage . | Eosinophil . | Megakaryocyte . | |||
| Total . | Multicentric . | Dispersed . | ||||||
| CD34− | ||||||||
| Erg+/+ | 18.4 ± 6.0 | 11.6 ± 4.6 | 6.9 ± 3.0 | 0.1 ± 0.4 | 0 | 3.9 ± 3.8 | 0 | 5.1 ± 6.2 |
| Erg+/Mld2 | 6.0 ± 3.8† | 4.0 ± 3.3† | 2.0 ± 1.9† | 0 | 0.1 ± 0.4 | 2.6 ± 2.4 | 0 | 8.1 ± 7.1 |
| CD34+Flt3R− | ||||||||
| Erg+/+ | 31.3 ± 8.1 | 27.3 ± 9.5 | 4.0 ± 1.7 | 3.9 ± 3.9 | 4.4 ± 3.8 | 4.0 ± 3.6 | 0 | 5.8 ± 2.0 |
| Erg+/Mld2 | 13.7 ± 10.5† | 12.3 ± 9.9† | 1.4 ± 2.1† | 3.3 ± 2.9 | 1.1 ± 0.9 | 0.7 ± 1.0 | 0.4 ± 0.8 | 5.1 ± 4.1 |
Cells were cultured for 7 days in granulocyte colony-stimulating factor (10 ng/mL) + stem cell factor (10 ng/mL) for enumeration of blast colonies or stem cell factor (10 ng/mL) + IL-3 (10 ng/mL) + erythropoietin (2 U/mL) for other colony types.
P < .022 after adjustment for multiple testing; n = 8 independent sorted cell samples per genotype.