Proteins with different degrees of Tyr phosphorylation in RBC membranes from control and ChAc subjects
| Band no. . | AC . | Protein . | Theoretical protein MW, kDa . | Matching peptide . | Coverage, % . |
|---|---|---|---|---|---|
| 1 | P11277 | β-spectrin (SPTB1) | 246.468 | 146 | 50 |
| 2 | P16157 | Ankyrin (ANK1) | 206.265 | 20 | 22 |
| 3 | Q8WX82 | β-I Spectrin form β I σ 3 (fragment; Q8WX82) | 117.549 | 14 | 20 |
| 4 | P02730 | Band 3 anion transport protein (B3AT) | 101.792 | 16 | 18 |
| 5 | P02730 | Band 3 anion transport protein (B3AT) | 101.792 | 23 | 24 |
| 6 | Q9H4B4 | Serine threonine protein kinase PLK3 ( PLK3) | 71.629 | 6 | 8 |
| 7 | P35612 | β-adducin (ADDB) | 80.854 | 9 | 11 |
| 8 | P11171 | Band 4.1 (EPB41) | 97.017 | 10 | 24 |
| 9 | P16452 | Band 4.2 (EPB42) | 77.009 | 9 | 12 |
| 10 | Q9H4B4 | Serine threonine protein kinase PLK3 ( PLK3) | 71.629 | 6 | 11 |
| 11 | Q00013 | 55-kDa erythrocyte membrane protein (EM55) | 52.296 | 19 | 34 |
| 12 | Q00013 | 55-kDa erythrocyte membrane protein (EM55) | 52.296 | 6 | 18 |
| 13 | P47895 | Aldehyde dehydrogenase family 1 member A3 (AL1A3) | 56.108 | 3 | 6 |
| 14 | P04040 | Catalase (CATA) | 59.756 | 4 | 9 |
| 15 | O94921 | Serine threonine protein kinase PFTAIRE-1 (CDK14) | 53.057 | 5 | 10 |
| 16 | Q14012 | Calcium calmodulin-dependent protein kinase type 1 (KCC1A) | 41.337 | 4 | 10 |
| 17 | P60709 | β-actin (ACTB) | 41.737 | 10 | 34 |
| 18 | Q96GD4 | Serine threonine protein kinase 12 (AURKB) | 39.311 | 4 | 15 |
| 19 | P04406 | Glyceraldehyde-3-P-dehydrogenase (G3P) | 36.053 | 9 | 36 |
| 20 | P27105 | Stomatin (STOM) | 31.731 | 10 | 40 |
| 21 | P00918 | Carbonic anhydrase 2 (CAH2) | 29.246 | 3 | 11 |
| 22 | Q96E17 | Ras-related protein Rab-3C (RAB3C) | 25.952 | 3 | 17 |
| Band no. . | AC . | Protein . | Theoretical protein MW, kDa . | Matching peptide . | Coverage, % . |
|---|---|---|---|---|---|
| 1 | P11277 | β-spectrin (SPTB1) | 246.468 | 146 | 50 |
| 2 | P16157 | Ankyrin (ANK1) | 206.265 | 20 | 22 |
| 3 | Q8WX82 | β-I Spectrin form β I σ 3 (fragment; Q8WX82) | 117.549 | 14 | 20 |
| 4 | P02730 | Band 3 anion transport protein (B3AT) | 101.792 | 16 | 18 |
| 5 | P02730 | Band 3 anion transport protein (B3AT) | 101.792 | 23 | 24 |
| 6 | Q9H4B4 | Serine threonine protein kinase PLK3 ( PLK3) | 71.629 | 6 | 8 |
| 7 | P35612 | β-adducin (ADDB) | 80.854 | 9 | 11 |
| 8 | P11171 | Band 4.1 (EPB41) | 97.017 | 10 | 24 |
| 9 | P16452 | Band 4.2 (EPB42) | 77.009 | 9 | 12 |
| 10 | Q9H4B4 | Serine threonine protein kinase PLK3 ( PLK3) | 71.629 | 6 | 11 |
| 11 | Q00013 | 55-kDa erythrocyte membrane protein (EM55) | 52.296 | 19 | 34 |
| 12 | Q00013 | 55-kDa erythrocyte membrane protein (EM55) | 52.296 | 6 | 18 |
| 13 | P47895 | Aldehyde dehydrogenase family 1 member A3 (AL1A3) | 56.108 | 3 | 6 |
| 14 | P04040 | Catalase (CATA) | 59.756 | 4 | 9 |
| 15 | O94921 | Serine threonine protein kinase PFTAIRE-1 (CDK14) | 53.057 | 5 | 10 |
| 16 | Q14012 | Calcium calmodulin-dependent protein kinase type 1 (KCC1A) | 41.337 | 4 | 10 |
| 17 | P60709 | β-actin (ACTB) | 41.737 | 10 | 34 |
| 18 | Q96GD4 | Serine threonine protein kinase 12 (AURKB) | 39.311 | 4 | 15 |
| 19 | P04406 | Glyceraldehyde-3-P-dehydrogenase (G3P) | 36.053 | 9 | 36 |
| 20 | P27105 | Stomatin (STOM) | 31.731 | 10 | 40 |
| 21 | P00918 | Carbonic anhydrase 2 (CAH2) | 29.246 | 3 | 11 |
| 22 | Q96E17 | Ras-related protein Rab-3C (RAB3C) | 25.952 | 3 | 17 |
The corresponding bands are indicated in Figure 2B. The success rate of protein identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry/mass spectrometry was 82% ± 6.7% (n = 9).
AC indicates accession number; and MW, molecular weight.