Gene expression after knockdown of NFATc1 or NFATc3
| Gene target . | Symbol . | KD-c1 . | KD-c3 . |
|---|---|---|---|
| Interleukin 2 | IL2 | 3.42 ± 0.15 | 0.45 ± 0.03 |
| Interleukin 3 | IL3 | 3.55 ± 0.33 | 0.98 ± 0.20 |
| Cyclooxygenase 2 | COX2 | 1.75 ± 0.34 | 0.29 ± 0.06 |
| Perforin 1 | PRF1 | 0.84 ± 0.08 | 0.74 ± 0.02 |
| TNF receptor member 6 | FAS | 1.22 ± 0.03 | 0.78 ± 0.18 |
| FAS ligand | FASLG | 1.89 ± 0.00 | 0.47 ± 0.09 |
| Transferrin receptor* | TFRC | 1.10 ± 0.00 | 1.05 ± 0.30 |
| Beta-glucuronidase* | GUSB | 1.07 ± 0.01 | 0.82 ± 0.09 |
| Gene target . | Symbol . | KD-c1 . | KD-c3 . |
|---|---|---|---|
| Interleukin 2 | IL2 | 3.42 ± 0.15 | 0.45 ± 0.03 |
| Interleukin 3 | IL3 | 3.55 ± 0.33 | 0.98 ± 0.20 |
| Cyclooxygenase 2 | COX2 | 1.75 ± 0.34 | 0.29 ± 0.06 |
| Perforin 1 | PRF1 | 0.84 ± 0.08 | 0.74 ± 0.02 |
| TNF receptor member 6 | FAS | 1.22 ± 0.03 | 0.78 ± 0.18 |
| FAS ligand | FASLG | 1.89 ± 0.00 | 0.47 ± 0.09 |
| Transferrin receptor* | TFRC | 1.10 ± 0.00 | 1.05 ± 0.30 |
| Beta-glucuronidase* | GUSB | 1.07 ± 0.01 | 0.82 ± 0.09 |
Jurkat cells transformed with lentiviral vectors encoding shRNAs for NFATc1 (KD-c1) or NFATc3 (KD-c3) were stimulated with PMA + Io for 4 hours. Total RNA was extracted and analyzed by gene expression array. Values correspond to the -fold induction (mean ± SD) relative to the expression in control cells transformed with scrambled shRNA (KDSc).
Endogenous control.