Absence of transplanted cells in organs and tissues away from the implants
| Organ/tissue . | ECFCs (hEPO/106 18S) . | MSCs (hCD90/106 18S) . | ||
|---|---|---|---|---|
| Implant-bearing . | Naive control . | Implant-bearing . | Naive control . | |
| Implant | 283.870 ± 107.641 | 2.545 ± 1.943 | ||
| Lung | 0.030 ± 0.012 | 0.025 ± 0.013 | 0.003 ± 0.005 | 0.000 ± 0.000 |
| Spleen | 0.023 ± 0.005 | 0.020 ± 0.014 | 0.003 ± 0.005 | 0.010 ± 0.008 |
| Kidney | 0.028 ± 0.010 | 0.025 ± 0.006 | 0.003 ± 0.005 | 0.000 ± 0.000 |
| Heart | 0.150 ± 0.096 | 0.110 ± 0.054 | 0.000 ± 0.000 | 0.008 ± 0.010 |
| Liver | 0.005 ± 0.006 | 0.013 ± 0.005 | 0.000 ± 0.000 | 0.000 ± 0.000 |
| Adipose tissue | 0.058 ± 0.030 | 0.050 ± 0.012 | 0.008 ± 0.010 | 0.005 ± 0.006 |
| Brain | 0.063 ± 0.013 | 0.068 ± 0.013 | 0.000 ± 0.000 | 0.005 ± 0.010 |
| BM | 0.055 ± 0.035 | 0.110 ± 0.000 | 0.003 ± 0.005 | 0.038 ± 0.017 |
| Blood | 0.008 ± 0.005 | 0.008 ± 0.010 | 0.003 ± 0.005 | 0.000 ± 0.000 |
| Organ/tissue . | ECFCs (hEPO/106 18S) . | MSCs (hCD90/106 18S) . | ||
|---|---|---|---|---|
| Implant-bearing . | Naive control . | Implant-bearing . | Naive control . | |
| Implant | 283.870 ± 107.641 | 2.545 ± 1.943 | ||
| Lung | 0.030 ± 0.012 | 0.025 ± 0.013 | 0.003 ± 0.005 | 0.000 ± 0.000 |
| Spleen | 0.023 ± 0.005 | 0.020 ± 0.014 | 0.003 ± 0.005 | 0.010 ± 0.008 |
| Kidney | 0.028 ± 0.010 | 0.025 ± 0.006 | 0.003 ± 0.005 | 0.000 ± 0.000 |
| Heart | 0.150 ± 0.096 | 0.110 ± 0.054 | 0.000 ± 0.000 | 0.008 ± 0.010 |
| Liver | 0.005 ± 0.006 | 0.013 ± 0.005 | 0.000 ± 0.000 | 0.000 ± 0.000 |
| Adipose tissue | 0.058 ± 0.030 | 0.050 ± 0.012 | 0.008 ± 0.010 | 0.005 ± 0.006 |
| Brain | 0.063 ± 0.013 | 0.068 ± 0.013 | 0.000 ± 0.000 | 0.005 ± 0.010 |
| BM | 0.055 ± 0.035 | 0.110 ± 0.000 | 0.003 ± 0.005 | 0.038 ± 0.017 |
| Blood | 0.008 ± 0.005 | 0.008 ± 0.010 | 0.003 ± 0.005 | 0.000 ± 0.000 |
The presence of ECFCs and MSCs was evaluated by quantitative RT-PCR measurements of hEPO/18S and hCD90/18S mRNA ratios, respectively. Implants, organs, and tissues from implant-bearing mice at 5 weeks were compared with those from naive control mice (n = 4; mean ± SD; see also supplemental Figure 12). Cells in culture had the following mRNA ratios: epoECFCs: hEPO/106 18S = 4808.43 and hCD90/106 18S = 0.040; MSCs: hEPO/106 18S = 0.010 and hCD90/106 18S = 33.660; and non-transfected ECFCs in culture: hEPO/106 18S = 0.000 and hCD90/106 18S = 0.020.