Table 1

Fold-change of gene expression associated with primitive hematopoiesis in metap2MO embryos at 18 hpf

GeneControlmetap2MOP value
scl 1.00 0.89 ± 0.07 .17 
lmo2 1.00 0.92 ± 0.08 .15 
gata1 1.00 1.24 ± 0.07 .17 
ehα1 1.00 1.14 ± 0.12 .43 
ehβ1 1.00 1.18 ± 0.08 .25 
pu.1 1.00 1.24 ± 0.05 .07 
l-plastin 1.00 0.99 ± 0.09 .25 
mpo 1.00 2.06 ± 0.10 .02 
GeneControlmetap2MOP value
scl 1.00 0.89 ± 0.07 .17 
lmo2 1.00 0.92 ± 0.08 .15 
gata1 1.00 1.24 ± 0.07 .17 
ehα1 1.00 1.14 ± 0.12 .43 
ehβ1 1.00 1.18 ± 0.08 .25 
pu.1 1.00 1.24 ± 0.05 .07 
l-plastin 1.00 0.99 ± 0.09 .25 
mpo 1.00 2.06 ± 0.10 .02 

Comparison of gene expression between control and the metap2MO embryos in each case was based on ΔCt as described previously. Statistical analysis was evaluated by the Student paired t test. Data as shown represent the average results of 3 experiments.

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