Effects of soluble Glu-Pg and Lys-Pg on the binding of anti-Pg mAbs to immobilized Glu-Pg
| . | Competitor, absorbance OD 405 . | ||
|---|---|---|---|
| mAb . | Buffer . | Glu-Pg . | Lys-Pg . |
| 20 | 0.76 ± 0.02 | 0.78 ± 0.05 | 0.78 ± 0.05 |
| 109 | 0.83 ± 0.06 | 0.82 ± 0.02 | 0.75 ± 0.06 |
| 49 | 0.57 ± 0.04 | 0.54 ± 0.01 | 0.66 ± 0.02 |
| 51 | 0.68 ± 0.03 | 0.60 ± 0.03 | 0.73 ± 0.06 |
| 53 | 0.69 ± 0.05 | 0.63 ± 0.03 | 0.69 ± 0.01 |
| 116 | 0.55 ± 0.03 | 0.50 ± 0.05 | 0.52 ± 0.02 |
| Buffer | 0.13 ± 0.01 | 0.13 ± 0.01 | 0.13 ± 0.01 |
| . | Competitor, absorbance OD 405 . | ||
|---|---|---|---|
| mAb . | Buffer . | Glu-Pg . | Lys-Pg . |
| 20 | 0.76 ± 0.02 | 0.78 ± 0.05 | 0.78 ± 0.05 |
| 109 | 0.83 ± 0.06 | 0.82 ± 0.02 | 0.75 ± 0.06 |
| 49 | 0.57 ± 0.04 | 0.54 ± 0.01 | 0.66 ± 0.02 |
| 51 | 0.68 ± 0.03 | 0.60 ± 0.03 | 0.73 ± 0.06 |
| 53 | 0.69 ± 0.05 | 0.63 ± 0.03 | 0.69 ± 0.01 |
| 116 | 0.55 ± 0.03 | 0.50 ± 0.05 | 0.52 ± 0.02 |
| Buffer | 0.13 ± 0.01 | 0.13 ± 0.01 | 0.13 ± 0.01 |
Binding of anti-Pg mAbs (180nM) to immobilized Glu-Pg was determined in the presence of either buffer, soluble Glu-Pg (1μM), or soluble Lys-Pg (1μM) as described in “ELISA for Glu-Pg.” Results are mean ± SEM; n = 3 for each experimental group.