The effect of catheter segments or tissue factor (RecombiPlasTin) on clotting times in normal or fVII-, fXI-, or fXII-deficient platelet-poor plasma
| Type of plasma/condition . | Time to clot, s . |
|---|---|
| Normal | |
| Control | 971 ± 92 |
| Catheter | 352 ± 22* |
| RecombiPlasTin | 102 ± 1* |
| Catheter + RecombiPlasTin | 93 ± 3* |
| fVII-deficient | |
| Control | 782 ± 35 |
| Catheter | 387 ± 27* |
| RecombiPlasTin | 514 ± 30* |
| Catheter + RecombiPlasTin | 320 ± 28* |
| fXI-deficient | |
| Control | > 2500 |
| Catheter | > 2500 |
| RecombiPlasTin | 136 ± 38* |
| Catheter + RecombiPlasTin | 140 ± 45* |
| f XII-deficient | |
| Control | 2467 ± 306 |
| Catheter | 1625 ± 532 |
| RecombiPlasTin | 133 ± 40* |
| Catheter + RecombiPlasTin | 131 ± 38* |
| Type of plasma/condition . | Time to clot, s . |
|---|---|
| Normal | |
| Control | 971 ± 92 |
| Catheter | 352 ± 22* |
| RecombiPlasTin | 102 ± 1* |
| Catheter + RecombiPlasTin | 93 ± 3* |
| fVII-deficient | |
| Control | 782 ± 35 |
| Catheter | 387 ± 27* |
| RecombiPlasTin | 514 ± 30* |
| Catheter + RecombiPlasTin | 320 ± 28* |
| fXI-deficient | |
| Control | > 2500 |
| Catheter | > 2500 |
| RecombiPlasTin | 136 ± 38* |
| Catheter + RecombiPlasTin | 140 ± 45* |
| f XII-deficient | |
| Control | 2467 ± 306 |
| Catheter | 1625 ± 532 |
| RecombiPlasTin | 133 ± 40* |
| Catheter + RecombiPlasTin | 131 ± 38* |
Plasma was incubated for 10 minutes at 37°C in the presence of PCI catheter segments before the addition of CaCl2 to 20mM with or without a 1/1500-fold dilution of RecombiPlasTin. As a control, clotting was monitored in the absence of catheters or RecombiPlasTin. Absorbance was monitored up to a maximum of 2500 seconds in a plate reader, and time to clot was determined as the time to one-half maximal absorbance. Results reflect the mean ± SD of ≥ 3 determinations.
P < .05 compared with the respective controls.