Engraftment of SHP2 shRNA-transduced human CD34+ cells in NSG mice
| Mouse ID . | shRNA . | Cell dose . | Time of assessment, wk . | % of CD45+ cells in BM . | No. of CD45+ cells in BM . |
|---|---|---|---|---|---|
| Control shRNA | |||||
| 1 | sh-Ctrla | 5.0 × 104 | 8 | 0.08 | 375 |
| 2 | sh-Ctrla | 6.0 × 104 | 8 | 2.32 | 31 960 |
| 3 | sh-Ctrla | 1.0 × 105 | 13 | 0.21 | 20 125 |
| 4 | sh-Ctrla | 1.0 × 105 | 13 | 0.46 | 25 739 |
| 5 | sh-Ctrla | 1.0 × 105 | 13 | 0.25 | 21 586 |
| 6 | sh-Ctrla | 1.0 × 105 | 13 | 0.04 | 2866 |
| SHP2 knockdown | |||||
| 7 | sh-Shp2-2 | 5.0 × 104 | 8 | 0 | 0 |
| 8 | sh-Shp2-2 | 5.0 × 104 | 8 | 0 | 0 |
| 9 | sh-Shp2-2 | 7.0 × 104 | 8 | 0.08 | 1863 |
| 10 | sh-Shp2-2 | 1.0 × 105 | 8 | 0.02 | 406 |
| 11 | sh-Shp2-2 | 1.0 × 105 | 8 | 0.06 | 1400 |
| 12 | sh-Shp2-2 | 1.0 × 105 | 8 | 0.03 | 1150 |
| 13 | sh-Shp2-2 | 1.0 × 105 | 8 | 0.04 | 851 |
| 14 | sh-Shp2-2 | 1.0 × 105 | 8 | 0.05 | 1223 |
| 15 | sh-Shp2-2 | 1.0 × 105 | 8 | 0.13 | 1602 |
| 16 | sh-Shp2-2 | 1.0 × 105 | 13 | 0 | 0 |
| 17 | sh-Shp2-2 | 1.0 × 105 | 13 | 0 | 0 |
| 18 | sh-Shp2-2 | 1.0 × 105 | 13 | 0.03 | 1524 |
| 19 | sh-Shp2-2 | 1.0 × 105 | 13 | 0.01 | 1573 |
| 20 | sh-Shp2-2 | 2.0 × 105 | 13 | 0 | 0 |
| 21 | sh-Shp2-2 | 2.0 × 105 | 13 | 0.14 | 15 969 |
| 22 | sh-Shp2-2 | 2.0 × 105 | 13 | 0.07 | 10 430 |
| 23 | sh-Shp2-2 | 2.0 × 105 | 13 | 0.03 | 2328 |
| 24 | sh-Shp2-2 | 2.0 × 105 | 13 | 0.06 | 4430 |
| Mouse ID . | shRNA . | Cell dose . | Time of assessment, wk . | % of CD45+ cells in BM . | No. of CD45+ cells in BM . |
|---|---|---|---|---|---|
| Control shRNA | |||||
| 1 | sh-Ctrla | 5.0 × 104 | 8 | 0.08 | 375 |
| 2 | sh-Ctrla | 6.0 × 104 | 8 | 2.32 | 31 960 |
| 3 | sh-Ctrla | 1.0 × 105 | 13 | 0.21 | 20 125 |
| 4 | sh-Ctrla | 1.0 × 105 | 13 | 0.46 | 25 739 |
| 5 | sh-Ctrla | 1.0 × 105 | 13 | 0.25 | 21 586 |
| 6 | sh-Ctrla | 1.0 × 105 | 13 | 0.04 | 2866 |
| SHP2 knockdown | |||||
| 7 | sh-Shp2-2 | 5.0 × 104 | 8 | 0 | 0 |
| 8 | sh-Shp2-2 | 5.0 × 104 | 8 | 0 | 0 |
| 9 | sh-Shp2-2 | 7.0 × 104 | 8 | 0.08 | 1863 |
| 10 | sh-Shp2-2 | 1.0 × 105 | 8 | 0.02 | 406 |
| 11 | sh-Shp2-2 | 1.0 × 105 | 8 | 0.06 | 1400 |
| 12 | sh-Shp2-2 | 1.0 × 105 | 8 | 0.03 | 1150 |
| 13 | sh-Shp2-2 | 1.0 × 105 | 8 | 0.04 | 851 |
| 14 | sh-Shp2-2 | 1.0 × 105 | 8 | 0.05 | 1223 |
| 15 | sh-Shp2-2 | 1.0 × 105 | 8 | 0.13 | 1602 |
| 16 | sh-Shp2-2 | 1.0 × 105 | 13 | 0 | 0 |
| 17 | sh-Shp2-2 | 1.0 × 105 | 13 | 0 | 0 |
| 18 | sh-Shp2-2 | 1.0 × 105 | 13 | 0.03 | 1524 |
| 19 | sh-Shp2-2 | 1.0 × 105 | 13 | 0.01 | 1573 |
| 20 | sh-Shp2-2 | 2.0 × 105 | 13 | 0 | 0 |
| 21 | sh-Shp2-2 | 2.0 × 105 | 13 | 0.14 | 15 969 |
| 22 | sh-Shp2-2 | 2.0 × 105 | 13 | 0.07 | 10 430 |
| 23 | sh-Shp2-2 | 2.0 × 105 | 13 | 0.03 | 2328 |
| 24 | sh-Shp2-2 | 2.0 × 105 | 13 | 0.06 | 4430 |