Sequence of primers used for real-time quantitative PCR with SYBR Green
| . | Forward . | Reverse . |
|---|---|---|
| Chemokine receptors | ||
| CX3CR1 | GGAGACTGGAGCCAACAGAG | CCTGATCCAGGGAATGCTAA |
| CCR1 | TGCAGGTGACTGAGGTGATTG | TGAAACAGCTGCCGAAGGTA |
| CCR2 | GGAAGACAATAATATGTTACCTCAGTT | TGGTGGCCCCTTCATCAA |
| CCR5 | CGAAAACACATGGTCAAACG | TTCCTACTCCCAAGCTGCAT |
| CXCR1 | CCCGATCCGTCATGGATGTC | CACAGGGGTGTGGCCAAAAATC |
| CXCR2 | ATGCCCTCTATTCTGCCAGAT | GTGCTCCGGTTGTATAAGATGAC |
| CXCR3 | CTCTTTGCCCTCCCAGATTTC | GGCATAGCAGTAGGCCATGA |
| CXCR4 | TCCAACAAGGAACCCTGCTTC | TTGCCGACTATGCCAGTCAAG |
| Chemokines | ||
| CX3CL1/fractalkine | TCCTGGAGACGACACAGCA | TGCCACCATTTTTAGTGAGGG |
| CCL2/MCP1 | TTAAAAACCTGGATCGGAACCAA | GCATTAGCTTCAGATTTACGGGT |
| CCL5/RANTES | GCTGCTTTGCCTACCTCTCC | TCGAGTGACAAACACGACTGC |
| CCL7/MCP3 | GGATCTCTGCCACGCTTCTGT | ACTTCCATGCCCTTCTTTGTCTTG |
| CXCL2/KC | TGAGCTGCGCTGTCAGTGCCT | AGAAGCCAGCGTTCACCAGA |
| CXCL5/LIX | GCATTTCTGTTGCTGTTCACGCTG | CCTCCTTCTGGTTTTTCAGTTTAGC |
| CXCL12/SDF1α | TGCATCAGTGACGGTAAACCA | TTCTTCAGCCGTGCAACAATC |
| TLRs | ||
| TLR2 | GCAAACGCTGTTCTGCTCAG | AGGCGTCTCCCTCTATTGTATT |
| TLR3 | GTGAGATACAACGTAGCTGACTG | TCCTGCATCCAAGATAGCAAGT |
| TLR4 | ATGGCATGGCTTACACCACC | GAGGCCAATTTTGTCTCCACA |
| TLR6 | TGAGCCAAGAACAGAAAACCCA | GGGACATGAGTAAGGTTCCTGTT |
| Myd88 | AGGACAAACGCCGGAACTTTT | GCCGATAGTCTGTCTGTTCTAGT |
| Rat TLR2 | CTCCTGTGAACTCCTGTCCTT | AGCTGTCTGGCCAGTCAAC |
| Rat TLR4 | CTGGGTTTCTGCTGTGGACA | AGGTTAGAAGCCTCGTGCTCC |
| Reference gene | ||
| 18S | AGCCTGCGGCTTAATTTGAC | CAACTAAGAACGGCCATGCA |
| . | Forward . | Reverse . |
|---|---|---|
| Chemokine receptors | ||
| CX3CR1 | GGAGACTGGAGCCAACAGAG | CCTGATCCAGGGAATGCTAA |
| CCR1 | TGCAGGTGACTGAGGTGATTG | TGAAACAGCTGCCGAAGGTA |
| CCR2 | GGAAGACAATAATATGTTACCTCAGTT | TGGTGGCCCCTTCATCAA |
| CCR5 | CGAAAACACATGGTCAAACG | TTCCTACTCCCAAGCTGCAT |
| CXCR1 | CCCGATCCGTCATGGATGTC | CACAGGGGTGTGGCCAAAAATC |
| CXCR2 | ATGCCCTCTATTCTGCCAGAT | GTGCTCCGGTTGTATAAGATGAC |
| CXCR3 | CTCTTTGCCCTCCCAGATTTC | GGCATAGCAGTAGGCCATGA |
| CXCR4 | TCCAACAAGGAACCCTGCTTC | TTGCCGACTATGCCAGTCAAG |
| Chemokines | ||
| CX3CL1/fractalkine | TCCTGGAGACGACACAGCA | TGCCACCATTTTTAGTGAGGG |
| CCL2/MCP1 | TTAAAAACCTGGATCGGAACCAA | GCATTAGCTTCAGATTTACGGGT |
| CCL5/RANTES | GCTGCTTTGCCTACCTCTCC | TCGAGTGACAAACACGACTGC |
| CCL7/MCP3 | GGATCTCTGCCACGCTTCTGT | ACTTCCATGCCCTTCTTTGTCTTG |
| CXCL2/KC | TGAGCTGCGCTGTCAGTGCCT | AGAAGCCAGCGTTCACCAGA |
| CXCL5/LIX | GCATTTCTGTTGCTGTTCACGCTG | CCTCCTTCTGGTTTTTCAGTTTAGC |
| CXCL12/SDF1α | TGCATCAGTGACGGTAAACCA | TTCTTCAGCCGTGCAACAATC |
| TLRs | ||
| TLR2 | GCAAACGCTGTTCTGCTCAG | AGGCGTCTCCCTCTATTGTATT |
| TLR3 | GTGAGATACAACGTAGCTGACTG | TCCTGCATCCAAGATAGCAAGT |
| TLR4 | ATGGCATGGCTTACACCACC | GAGGCCAATTTTGTCTCCACA |
| TLR6 | TGAGCCAAGAACAGAAAACCCA | GGGACATGAGTAAGGTTCCTGTT |
| Myd88 | AGGACAAACGCCGGAACTTTT | GCCGATAGTCTGTCTGTTCTAGT |
| Rat TLR2 | CTCCTGTGAACTCCTGTCCTT | AGCTGTCTGGCCAGTCAAC |
| Rat TLR4 | CTGGGTTTCTGCTGTGGACA | AGGTTAGAAGCCTCGTGCTCC |
| Reference gene | ||
| 18S | AGCCTGCGGCTTAATTTGAC | CAACTAAGAACGGCCATGCA |