Table 1 Purification of Tregs from human... | American Society of Hematology

Table 1

Purification of Tregs from human blood

Step	Cell type	Total live cells	CD4⁺	Tregs	Treg yield, %
Step	Cell type	Millions			Treg yield, %
1	PBMCs	100	24.2 ± 9.4	1.96 ± 0.8
2	CD4⁺/CD127⁻	19 ± 12	5.8 ± 7.3	1.55 ± 1.1
3	CD25⁺ (CD4⁺CD127⁻)	0.61 ± 0.3	0.60 ± 8	0.59 ± 0.3	29.5 ± 4

Step	Cell type	Total live cells	CD4⁺	Tregs	Treg yield, %
Step	Cell type	Millions			Treg yield, %
1	PBMCs	100	24.2 ± 9.4	1.96 ± 0.8
2	CD4⁺/CD127⁻	19 ± 12	5.8 ± 7.3	1.55 ± 1.1
3	CD25⁺ (CD4⁺CD127⁻)	0.61 ± 0.3	0.60 ± 8	0.59 ± 0.3	29.5 ± 4

The total numbers of starting PBMCs were normalized to 100 million. Tregs were isolated using the CD4⁺CD25⁺CD127⁻ Treg isolation kit II magnetic MicroBead method (Miltenyi Biotec). Data represent the yield of purified Tregs from normal human buffy coat cells. Shown are the means ± SD of 8 independent experiments.

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