Time to carotid artery occlusion with FeCl3-induced injury
| Group . | Genotype* . | Sample . | Dose (µg/kg) . | No. of mice . | Time to occlusion (min) . | |||
|---|---|---|---|---|---|---|---|---|
| Total . | No occlusion . | Transient occlusion . | Complete occlusion . | |||||
| Control | WT | PBS | – | 7 | 0 | 0 | 7 | 15 ± 0.5 |
| Control | HB | PBS | – | 7 | 7 | 0 | 0 | >30 |
| Experimental, 10 minutes after injury | ||||||||
| Wild-type | HB | wt-FXa | 450 | 5 | 5 | 0 | 0 | >30 |
| Group 1 | HB | FXa-V17M | 450 | 7 | 0 | 1 | 6 | 3.2 ± 0.3 |
| Group 2 | HB | FXa-I16M | 450 | 7 | 0 | 0 | 7 | 2.0 ± 0.1 |
| Group 3 | HB | FXa-I16T | 450 | 9 | 0 | 0 | 9 | 3.3 ± 0.3 |
| Experimental, 15 minutes before injury | ||||||||
| Group 1 | HB | FXa-V17M | 450 | 7 | 7 | 0 | 0 | >30 |
| Group 2 | HB | FXa-I16M | 450 | 7 | 6 | 0 | 1 | 7.1 |
| Group 3 | HB | FXa-I16T | 450 | 9 | 1 | 0 | 8 | 7.6 ± 1.2 |
| HB | FXa-I16T | 225 | 7 | 2 | 0 | 5 | 12 ± 0.7 | |
| HB | FXa-I16T | 113 | 7 | 2 | 0 | 5 | 13 ± 1 | |
| HB | FXa-I16T | 56 | 5 | 4 | 0 | 1 | 12 | |
| Group . | Genotype* . | Sample . | Dose (µg/kg) . | No. of mice . | Time to occlusion (min) . | |||
|---|---|---|---|---|---|---|---|---|
| Total . | No occlusion . | Transient occlusion . | Complete occlusion . | |||||
| Control | WT | PBS | – | 7 | 0 | 0 | 7 | 15 ± 0.5 |
| Control | HB | PBS | – | 7 | 7 | 0 | 0 | >30 |
| Experimental, 10 minutes after injury | ||||||||
| Wild-type | HB | wt-FXa | 450 | 5 | 5 | 0 | 0 | >30 |
| Group 1 | HB | FXa-V17M | 450 | 7 | 0 | 1 | 6 | 3.2 ± 0.3 |
| Group 2 | HB | FXa-I16M | 450 | 7 | 0 | 0 | 7 | 2.0 ± 0.1 |
| Group 3 | HB | FXa-I16T | 450 | 9 | 0 | 0 | 9 | 3.3 ± 0.3 |
| Experimental, 15 minutes before injury | ||||||||
| Group 1 | HB | FXa-V17M | 450 | 7 | 7 | 0 | 0 | >30 |
| Group 2 | HB | FXa-I16M | 450 | 7 | 6 | 0 | 1 | 7.1 |
| Group 3 | HB | FXa-I16T | 450 | 9 | 1 | 0 | 8 | 7.6 ± 1.2 |
| HB | FXa-I16T | 225 | 7 | 2 | 0 | 5 | 12 ± 0.7 | |
| HB | FXa-I16T | 113 | 7 | 2 | 0 | 5 | 13 ± 1 | |
| HB | FXa-I16T | 56 | 5 | 4 | 0 | 1 | 12 | |
In HB mice, protein was infused 10 minutes after or 15 minutes before 7.5% FeCl3 exposure. Data for mice presenting with no occlusion, transient occlusion, or complete occlusion of the carotid artery following FeCl3 exposure are provided. Occlusion time measurements are presented as mean ± SEM and are derived from both transient and complete events.
HB, hemophilia B mice; WT, hemostatically normal mice.