Summary of PACSIN2 F-BAR domain membrane tubulation in vitro
| GST-PACSIN2 F-BAR domain . | His-FlnA . | Membrane tubulation . |
|---|---|---|
| WT | None | Yes |
| WT | Full length | Yes, enhanced |
| WT | Repeats 1-8+24 | Yes |
| WT | Repeats 8-15+24 | Yes |
| WT | Repeats 16-24 | Yes, enhanced |
| P180A | None | Yes |
| P180A | Full length | Yes |
| None | Full length | No |
| GST-PACSIN2 F-BAR domain . | His-FlnA . | Membrane tubulation . |
|---|---|---|
| WT | None | Yes |
| WT | Full length | Yes, enhanced |
| WT | Repeats 1-8+24 | Yes |
| WT | Repeats 8-15+24 | Yes |
| WT | Repeats 16-24 | Yes, enhanced |
| P180A | None | Yes |
| P180A | Full length | Yes |
| None | Full length | No |
PC/PS liposomes (0.2 mg/mL) were incubated or not with GST-PACSIN2 F-BAR domain WT or mutant P180A (2 µM) in the presence or absence of His-FlnA constructs (400 nM), as indicated.