Validation of primary Treg transcriptome study by confirmatory microarray experiments
| Gene symbol . | Gene title . | GVHD vs no GVHD < 100 days . | GVHD vs no GVHD > 100 days . | Probe set ID . | Unigene . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| FC1 . | FC2 . | FC3 . | FC4 . | FC5 . | FC6 . | FC7 . | FC8 . | ||||
| CDKN2B | Cyclin-dependent kinase inhibitor 2B | 2.8 | 3.6 | 1.6 | 3.2 | NR | 1.7 | NR | 1.8 | 236313_at | Hs.729 01 |
| CDK6 | Cyclin-dependent kinase 6 | 2.4 | 2.3 | NR | 2.8 | 1.5 | NR | NR | NR | 224851_at | Hs.119882 |
| LAG3 | Lymphocyte-activation gene 3 | −2.3 | NR | NR | −2.1 | −1.9 | −1.7 | −1.8 | −1.6 | 206486_at | Hs.409523 |
| CD44 | CD44 molecule (Indian blood group) | −2.9 | NR | −1.6 | −4.4 | −3.4 | NR | −3.3 | NR | 204490_s_at | Hs.502328 |
| GZMA | Granzyme A | −3.4 | −2.7 | −8.0 | −2.9 | NR | −2.1 | NR | −1.9 | 205488_at | Hs.907 08 |
| CXCR6 | Chemokine (C-X-C motif) receptor 6 | −3.8 | 1.9 | 3.2 | −2.0 | NR | NR | −1.5 | NR | 206974_at | Hs.345 26 |
| LGALS1 | Galectin-1 | −1.9 | NR | NR | −2.8 | −1.8 | NR | −1.8 | NR | 201105_at | Hs.445351 |
| CCR5 | Chemokine (C-C motif) receptor 5 | −4.4 | NR | NR | −1.9 | NR | NR | NR | NR | 206991_s_at | Hs.536735 |
| CCR3 | Chemokine (C-C motif) receptor 3 | −5.7 | NR | 1.9 | −1.6 | 1.6 | 1.5 | NR | NR | 208304_at | Hs.506190 |
| CCR1 | Chemokine (C-C motif) receptor 1 | −6.4 | −5.8 | −19.6 | −1.5 | NR | 2.7 | NR | 1.7 | 205098_at | Hs.301921 |
| CXCR3 | Chemokine (C-X-C motif) receptor 3 | −6.3 | NR | NR | −2.3 | −1.5 | NR | −1.6 | NR | 207681_at | Hs.198252 |
| Gene symbol . | Gene title . | GVHD vs no GVHD < 100 days . | GVHD vs no GVHD > 100 days . | Probe set ID . | Unigene . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| FC1 . | FC2 . | FC3 . | FC4 . | FC5 . | FC6 . | FC7 . | FC8 . | ||||
| CDKN2B | Cyclin-dependent kinase inhibitor 2B | 2.8 | 3.6 | 1.6 | 3.2 | NR | 1.7 | NR | 1.8 | 236313_at | Hs.729 01 |
| CDK6 | Cyclin-dependent kinase 6 | 2.4 | 2.3 | NR | 2.8 | 1.5 | NR | NR | NR | 224851_at | Hs.119882 |
| LAG3 | Lymphocyte-activation gene 3 | −2.3 | NR | NR | −2.1 | −1.9 | −1.7 | −1.8 | −1.6 | 206486_at | Hs.409523 |
| CD44 | CD44 molecule (Indian blood group) | −2.9 | NR | −1.6 | −4.4 | −3.4 | NR | −3.3 | NR | 204490_s_at | Hs.502328 |
| GZMA | Granzyme A | −3.4 | −2.7 | −8.0 | −2.9 | NR | −2.1 | NR | −1.9 | 205488_at | Hs.907 08 |
| CXCR6 | Chemokine (C-X-C motif) receptor 6 | −3.8 | 1.9 | 3.2 | −2.0 | NR | NR | −1.5 | NR | 206974_at | Hs.345 26 |
| LGALS1 | Galectin-1 | −1.9 | NR | NR | −2.8 | −1.8 | NR | −1.8 | NR | 201105_at | Hs.445351 |
| CCR5 | Chemokine (C-C motif) receptor 5 | −4.4 | NR | NR | −1.9 | NR | NR | NR | NR | 206991_s_at | Hs.536735 |
| CCR3 | Chemokine (C-C motif) receptor 3 | −5.7 | NR | 1.9 | −1.6 | 1.6 | 1.5 | NR | NR | 208304_at | Hs.506190 |
| CCR1 | Chemokine (C-C motif) receptor 1 | −6.4 | −5.8 | −19.6 | −1.5 | NR | 2.7 | NR | 1.7 | 205098_at | Hs.301921 |
| CXCR3 | Chemokine (C-X-C motif) receptor 3 | −6.3 | NR | NR | −2.3 | −1.5 | NR | −1.6 | NR | 207681_at | Hs.198252 |
Differential gene expression changes for regulated gene transcripts in the primary and confirmatory Treg transcriptome study are presented as fold change (FC) comparing GVHD patients with immune tolerant (no GVHD) patients early (< 100 days) and late (> 100 days) after SCT.
NR indicates not regulated.