Effect of Rps6 hemizygosity and Trp53 mutations on BM cell counts*
| . | Tg.MxCre/+ . | Rps6lox/+; Tg.MxCre/+ . | Rps6lox/+;Tg.MxCre/+; Trp53ko/ko . |
|---|---|---|---|
| Immature erythroid* | 13.50 ± 1.66 | 40.00 ± 3.24a† | 13.67 ± 3.71a‡, NS§ |
| Mature erythroid* | 37.5 ± 1.26 | 21.0 ± 3.72b† | 39.0 ± 4.16b‡, NS§ |
| Lymphocyte | 70.75 ± 6.02 | 29.75 ± 5.02a† | 52.00 ± 1.73a‡b, § |
| Myeloblast | 2.5 ± 0.65 | 3.25 ± 0.48 | 4.67 ± 1.67 |
| Immature myeloid* | 19.75 ± 0.75 | 21.00 ± 1.96 | 19.67 ± 3.53 |
| Mature myeloid* | 93.75 ± 1.89 | 121.75 ± 7.22 | 108.00 ± 6.93 |
| Monocyte/macrophage | 20 ± 3.87 | 22.25 ± 3.25 | 20.33 ± 3.53 |
| Cellularity (× 107)‖ | 7.59 ± 0.42 | 3.56 ± 0.28c† | 6.39 ± 0.61b‡, NS§ |
| . | Tg.MxCre/+ . | Rps6lox/+; Tg.MxCre/+ . | Rps6lox/+;Tg.MxCre/+; Trp53ko/ko . |
|---|---|---|---|
| Immature erythroid* | 13.50 ± 1.66 | 40.00 ± 3.24a† | 13.67 ± 3.71a‡, NS§ |
| Mature erythroid* | 37.5 ± 1.26 | 21.0 ± 3.72b† | 39.0 ± 4.16b‡, NS§ |
| Lymphocyte | 70.75 ± 6.02 | 29.75 ± 5.02a† | 52.00 ± 1.73a‡b, § |
| Myeloblast | 2.5 ± 0.65 | 3.25 ± 0.48 | 4.67 ± 1.67 |
| Immature myeloid* | 19.75 ± 0.75 | 21.00 ± 1.96 | 19.67 ± 3.53 |
| Mature myeloid* | 93.75 ± 1.89 | 121.75 ± 7.22 | 108.00 ± 6.93 |
| Monocyte/macrophage | 20 ± 3.87 | 22.25 ± 3.25 | 20.33 ± 3.53 |
| Cellularity (× 107)‖ | 7.59 ± 0.42 | 3.56 ± 0.28c† | 6.39 ± 0.61b‡, NS§ |
NS indicates not significant.
BM cytology was evaluated from cytospins from 4 nonmutant, 4 Rps6lox/+;Tg.MxCre/+, and 3 Rps6lox/+;Tg.MxCre/+;Trp53ko/ko animals. A total of 260 cells were evaluated per animal. Immature erythroid cells are Pre CFU-E or CFU-E cells as defined by Pronk et al21 (supplemental Table 4); mature erythroid cells are either basophilic, polychromatic or orthochromatic erythroblasts22 (supplemental Table 4); immature myeloid cells are promyelocytes or myelocytes; and mature myeloid cells are band or segmented neutrophils. The number of cells within each population is given as mean ± SEM with P values on the basis of 2-tailed Student t test, aP < .01, bP < .05, cP < .001.
Nonmutant versus Rps6lox/+;Tg.MxCre/+.
Rps6lox/+;Tg.MxCre/+ vs Rps6lox/+;Tg.MxCre/+;Trp53ko/ko.
Nonmutant vs Rps6lox/+;Tg.MxCre/+;Trp53ko/ko.
BM cellularity determined by flushing 2 femurs and 2 tibias from 7 nonmutant, 7 Rps6lox/+;Tg.MxCre/+, and 3 Rps6lox/+;Tg.MxCre/+;Trp53ko/ko animals. Values are given as mean ± SEM with P values based on 2-tailed Student t test, aP < .01, bP < .05, cP < .001.