Primers for quantitative PCR
| mRNA . | Forward primer (5′- > 3′) . | Reverse primer (5′- > 3′) . |
|---|---|---|
| Mouse | ||
| Atf4 | GAGCTTCCTGAACAGCGAAGTG | TGGCCACCTCCAGATAGTCATC |
| Bsp | AAGAAGAGGAAGAGGAAGAAAATGAGAACGA | GCTTCTTCTCCGTTGTCTCC |
| DKK1 | GATATCCCAGAAGAACCACACTGACT | GGACCAGAAGTGTCTTGCACAA |
| Gapdh | CAGTGCCAGCCTCGTCCCGTAGA | CTGCAAATGGCAGCCCTGGTGAC |
| Gfi1 | AGGAGGCACCGAGAGACTCA | GGGAGGCAGGGAAGACATC |
| IL-7 | GTGCTGCTCGCAAGTTGAAG | AGTTCACCAGTGTTTGTGTGC |
| Ocn | TAGTGAACAGACTCCGGCGCTA | TGTAGGCGGTCTTCAAGCCAT |
| Osx | AGAGGTTCACTCGCTCTGACGA | TTGCTCAAGTGGTCGCTTCTG |
| Runx2 | TAAAGTGACAGTGGACGGTCCC | TGCGCCCTAAATCACTGAGG |
| TNF-α | CCCTCACACTCAGATCATCTTCT | GCTACGACGTGGGCTACAG |
| Human | ||
| Bsp | TGCCTTGAGCCTGCTTCC | GCAAAATTAAAGCAGTCTTCATTTTG |
| Gapdh | GAAGGTGAAGGTCGGAGTC | GAAGATGGTGATGGGATTTC |
| Gfi1 | GAGCCTGGAGCAGCACAAAG | GTGGATGACCTCTTGAAGCTCTTC |
| Ocn | GAAGCCCAGCGGTGCA | CACTACCTCGCTGCCCTCC |
| Runx2 | GCCTTCAAGGTGGTAGCCC | CGTTACCCGCCATGACAGTA |
| mRNA . | Forward primer (5′- > 3′) . | Reverse primer (5′- > 3′) . |
|---|---|---|
| Mouse | ||
| Atf4 | GAGCTTCCTGAACAGCGAAGTG | TGGCCACCTCCAGATAGTCATC |
| Bsp | AAGAAGAGGAAGAGGAAGAAAATGAGAACGA | GCTTCTTCTCCGTTGTCTCC |
| DKK1 | GATATCCCAGAAGAACCACACTGACT | GGACCAGAAGTGTCTTGCACAA |
| Gapdh | CAGTGCCAGCCTCGTCCCGTAGA | CTGCAAATGGCAGCCCTGGTGAC |
| Gfi1 | AGGAGGCACCGAGAGACTCA | GGGAGGCAGGGAAGACATC |
| IL-7 | GTGCTGCTCGCAAGTTGAAG | AGTTCACCAGTGTTTGTGTGC |
| Ocn | TAGTGAACAGACTCCGGCGCTA | TGTAGGCGGTCTTCAAGCCAT |
| Osx | AGAGGTTCACTCGCTCTGACGA | TTGCTCAAGTGGTCGCTTCTG |
| Runx2 | TAAAGTGACAGTGGACGGTCCC | TGCGCCCTAAATCACTGAGG |
| TNF-α | CCCTCACACTCAGATCATCTTCT | GCTACGACGTGGGCTACAG |
| Human | ||
| Bsp | TGCCTTGAGCCTGCTTCC | GCAAAATTAAAGCAGTCTTCATTTTG |
| Gapdh | GAAGGTGAAGGTCGGAGTC | GAAGATGGTGATGGGATTTC |
| Gfi1 | GAGCCTGGAGCAGCACAAAG | GTGGATGACCTCTTGAAGCTCTTC |
| Ocn | GAAGCCCAGCGGTGCA | CACTACCTCGCTGCCCTCC |
| Runx2 | GCCTTCAAGGTGGTAGCCC | CGTTACCCGCCATGACAGTA |