Enzyme-blocking activity of anti-MMP12 antibody
| Conditioned media . | Enzymatic activity, pg/mL . |
|---|---|
| Control* | 84.2 ± 11.6 (100%) |
| Control + anti-MMP12† | 51.2 ± 10.5 (60.8% of control) |
| pMMP12‡ | 131.4 ± 12.1 (156.05% of control) |
| pMMP12 + anti-MMP12§ | 91.3 ± 10.8 (108.4% of control) |
| Conditioned media . | Enzymatic activity, pg/mL . |
|---|---|
| Control* | 84.2 ± 11.6 (100%) |
| Control + anti-MMP12† | 51.2 ± 10.5 (60.8% of control) |
| pMMP12‡ | 131.4 ± 12.1 (156.05% of control) |
| pMMP12 + anti-MMP12§ | 91.3 ± 10.8 (108.4% of control) |
MMP-12 enzymatic activity was measured in ECFC conditioned medium using a fluorimetric kit (EnzoLyte 520 MMP-12 assay kit with 5-FAM/QXL 520 fluorescence resonance energy transfer; AnaSpec, DBA-Italia), as previously described.11
ECFC conditioned medium under basal conditions.
ECFC conditioned medium under basal conditions in the presence of anti-MMP12 antibody.
Conditioned medium from MMP12 transiently transfected ECFCs.
Medium from MMP12 transiently transfected ECFCs in the presence of anti-MMP12 antibody.