PCR primers and cycling conditions
| Primers . | Sequence . | Size, bp . | Cycling profile . |
|---|---|---|---|
| uPAR | 5′-AAAATGCTGTGTGCTGCTGACC-3′(sense) 5′-CCCTGCCCTGAAGTCGTTAGTG-3′ (antisense) | 910 | 94°C, 1 minute 56°C, 1 minute 72°C, 1 minute 35 cycles total |
| Caveolin-1 | 5′-CAACATCTACAAGCCCA-3′(sense) 5′-AAA CTT CTA CAC TAA CG-3′ (antisense) | 146 | 94°C, 30 seconds 50°C, 30 seconds 70°C, 30 seconds 30 cycles total |
| MMP-12 | 5′-CCACTGCTTCTGAGCTCTT-3′(sense) 5′-GCGTAGTCAACATCCTCACG-3′(antisense) | 350 | 94°C, 1 minute 58°C, 1 minute 72°C, 1 minute 35 cycles total |
| GAPDH | 5′-CCACCCATGGCAAATTCCATGGCA-3′(sense) 5′-TCTAGACGGCAGGTCAGGTCCACC-3′(antisense) | 598 | 94°C, 1 minute 56°C, 1 minute 72°C, 1 minute 35 cycles total |
| Primers . | Sequence . | Size, bp . | Cycling profile . |
|---|---|---|---|
| uPAR | 5′-AAAATGCTGTGTGCTGCTGACC-3′(sense) 5′-CCCTGCCCTGAAGTCGTTAGTG-3′ (antisense) | 910 | 94°C, 1 minute 56°C, 1 minute 72°C, 1 minute 35 cycles total |
| Caveolin-1 | 5′-CAACATCTACAAGCCCA-3′(sense) 5′-AAA CTT CTA CAC TAA CG-3′ (antisense) | 146 | 94°C, 30 seconds 50°C, 30 seconds 70°C, 30 seconds 30 cycles total |
| MMP-12 | 5′-CCACTGCTTCTGAGCTCTT-3′(sense) 5′-GCGTAGTCAACATCCTCACG-3′(antisense) | 350 | 94°C, 1 minute 58°C, 1 minute 72°C, 1 minute 35 cycles total |
| GAPDH | 5′-CCACCCATGGCAAATTCCATGGCA-3′(sense) 5′-TCTAGACGGCAGGTCAGGTCCACC-3′(antisense) | 598 | 94°C, 1 minute 56°C, 1 minute 72°C, 1 minute 35 cycles total |