Effects of Notch receptors and Ligands blockade on B-ALL cell survival
| Treatment . | B-ALL in coculture with hBM-MSCs (% of live cells) . | B-ALL in culture alone (% of live cells) . |
|---|---|---|
| No treatment | 70.2 (4.1) | 46.3 (3.9)* |
| Anti-Notch-1 | 64.6 (3.5)† | 43.0 (5.3) |
| Anti-Notch-3 | 50.9 (6.3)* | 26.0 (6.4)* |
| Anti-Notch-4 | 29.4 (13.0)* | 15.4 (5.3)* |
| Anti-Notch-1/-3/-4 | 35.1 (6.8)* | 11.8 (2.6)* |
| Anti-Notch-3/-4 | 36.0 (6.2)* | 11.3 (3.0)* |
| Anti-Jagged-1 | 46.7 (5.4)* | 26.6 (1.8)* |
| Anti-Jagged-2 | 49.7 (4.7)* | 32.5 (3.4)* |
| Anti-DLL-1 | 45.5 (8.2)* | 22.1 (4.7)* |
| Anti-DLL-3 | 65.4 (4.9) | 42.5 (2.9) |
| Anti-DLL-4 | 63.5 (6.8) | 41.1 (3.2) |
| Anti-Jagged-1/-2, DLL-1 | 36.3 (3.6)* | 12.7 (1.8)* |
| Anti-Jagged-1/-2, DLL-1/-3 | 38.2 (3.0)* | 12.2 (1.3)* |
| Anti-Jagged-1/-2, DLL-1/-4 | 34.4 (5.4)* | 12.8 (1.6)* |
| Anti-Jagged-1/-2, DLL-1/-3/-4 | 36.8 (6.4)* | 11.5 (2.1)* |
| Anti-DLL-3/-4 | 61.7 (5.1) | 43.9 (3.5) |
| GSI XII | 39.3 (4.2)* | 12.5 (5.2)* |
| DMSO | 65.9 (7.9) | 42.2 (2.7) |
| Treatment . | B-ALL in coculture with hBM-MSCs (% of live cells) . | B-ALL in culture alone (% of live cells) . |
|---|---|---|
| No treatment | 70.2 (4.1) | 46.3 (3.9)* |
| Anti-Notch-1 | 64.6 (3.5)† | 43.0 (5.3) |
| Anti-Notch-3 | 50.9 (6.3)* | 26.0 (6.4)* |
| Anti-Notch-4 | 29.4 (13.0)* | 15.4 (5.3)* |
| Anti-Notch-1/-3/-4 | 35.1 (6.8)* | 11.8 (2.6)* |
| Anti-Notch-3/-4 | 36.0 (6.2)* | 11.3 (3.0)* |
| Anti-Jagged-1 | 46.7 (5.4)* | 26.6 (1.8)* |
| Anti-Jagged-2 | 49.7 (4.7)* | 32.5 (3.4)* |
| Anti-DLL-1 | 45.5 (8.2)* | 22.1 (4.7)* |
| Anti-DLL-3 | 65.4 (4.9) | 42.5 (2.9) |
| Anti-DLL-4 | 63.5 (6.8) | 41.1 (3.2) |
| Anti-Jagged-1/-2, DLL-1 | 36.3 (3.6)* | 12.7 (1.8)* |
| Anti-Jagged-1/-2, DLL-1/-3 | 38.2 (3.0)* | 12.2 (1.3)* |
| Anti-Jagged-1/-2, DLL-1/-4 | 34.4 (5.4)* | 12.8 (1.6)* |
| Anti-Jagged-1/-2, DLL-1/-3/-4 | 36.8 (6.4)* | 11.5 (2.1)* |
| Anti-DLL-3/-4 | 61.7 (5.1) | 43.9 (3.5) |
| GSI XII | 39.3 (4.2)* | 12.5 (5.2)* |
| DMSO | 65.9 (7.9) | 42.2 (2.7) |
Values are percentages of overall live human B-ALL cells (mean ± SD of 10 independent experiments); DMSO is the vehicle control for GSI XII, 10μM.
B-ALL indicates B-lineage acute lymphoblastic leukemia; hBM-MSC, human bone marrow–mesenchymal stromal cell; GSI, γ-secretase inhibitor; Anti-, blocking Ab; and DLL, Delta-like ligand.
P < .001 (statistical analysis by 1-way ANOVA, Holm-Sidak test).
P < .05 (statistical analysis by 1-way ANOVA, Holm-Sidak test).
P < .01 (statistical analysis by 1-way ANOVA, Holm-Sidak test).