Expression of Notch receptors and ligands in B-ALL cells and hBM-MSCs in different culture conditions
| . | Jagged-1 . | Jagged-2 . | DLL-1 . | DLL-3 . | DLL-4 . | Notch-1 . | Notch-2 . | Notch-3 . | Notch-4 . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Day 3 . | Day 7 . | Day 3 . | Day 7 . | Day 3 . | Day 7 . | Day 3 . | Day 7 . | Day 3 . | Day 7 . | Day 3 . | Day 7 . | Day 3 . | Day 7 . | Day 3 . | Day 7 . | Day 3 . | Day 7 . | |
| B-ALL cells | ||||||||||||||||||
| Alone | 32.7 ± 2.2 | 19.0 ± 1.6 | 31.4 ± 9.0 | 26.6 ± 8.1 | 0.2 ± 0.0 | 26.9 ± 2.6 | 34.0 ± 5.7 | 32.1 ± 4.9 | 45.0 ± 2.0 | 47.7 ± 3.1 | 1.5 ± 0.6 | 1.9 ± 0.2 | 0.9 ± 0.1 | 0.2 ± 0.0 | 24.8 ± 0.6 | 17.5 ± 0.2 | 4.9 ± 2.1 | 1.7 ± 0.9 |
| Coculture | 52.4 ± 5.4 | 28.4 ± 3.9 | 36.0 ± 5.4 | 29.8 ± 4.0 | 0.6 ± 0.2 | 39.8 ± 4.1 | 38.4 ± 6.9 | 36.3 ± 3.8 | 49.4 ± 5.8 | 48.4 ± 1.5 | 28.3 ± 2.8 | 1.1 ± 0.4 | 0.4 ± 0.0 | 0.7 ± 0.5 | 35.3 ± 2.2 | 27.2 ± 3.3 | 9.6 ± 1.8 | 6.6 ± 2.5 |
| Student t test | P < .01 | P < .05 | NS | NS | NS | P < .01 | NS | NS | NS | NS | P < .001 | NS | NS | NS | P < .05 | P < .05 | P < .05 | P < .05 |
| hBM-MSCs | ||||||||||||||||||
| Alone | 0.7 ± 0.1 | 0.9 ± 0.0 | 1.2 ± 0.3 | 1.6 ± 1.7 | 0.3 ± 0.0 | 0.4 ± 0.0 | 31.8 ± 0.3 | 32.4 ± 0.5 | 7.1 ± 1.4 | 6.5 ± 2.3 | 7.2 ± 0.2 | 6.5 ± 0.7 | 0.7 ± 0.1 | 0.5 ± 0.1 | 30.6 ± 5.3 | 26.3 ± 1.7 | 1.6 ± 0.2 | 3.1 ± 0.7 |
| Coculture | 1.3 ± 0.4 | 1.6 ± 0.7 | 1.9 ± 0.5 | 1.1 ± 1.4 | 0.5 ± 0.0 | 0.3 ± 0.0 | 34.3 ± 0.1 | 35.6 ± 0.8 | 7.9 ± 1.9 | 8.6 ± 1.7 | 8.9 ± 0.7 | 8.1 ± 0.4 | 0.5 ± 0.0 | 0.4 ± 0.0 | 51.3 ± 2.2 | 37.2 ± 5.7 | 8.0 ± 4.1 | 9.1 ± 3.6 |
| Student t test | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | P < 0.01 | P < 0.05 | P < 0.05 | P < 0.05 |
| . | Jagged-1 . | Jagged-2 . | DLL-1 . | DLL-3 . | DLL-4 . | Notch-1 . | Notch-2 . | Notch-3 . | Notch-4 . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Day 3 . | Day 7 . | Day 3 . | Day 7 . | Day 3 . | Day 7 . | Day 3 . | Day 7 . | Day 3 . | Day 7 . | Day 3 . | Day 7 . | Day 3 . | Day 7 . | Day 3 . | Day 7 . | Day 3 . | Day 7 . | |
| B-ALL cells | ||||||||||||||||||
| Alone | 32.7 ± 2.2 | 19.0 ± 1.6 | 31.4 ± 9.0 | 26.6 ± 8.1 | 0.2 ± 0.0 | 26.9 ± 2.6 | 34.0 ± 5.7 | 32.1 ± 4.9 | 45.0 ± 2.0 | 47.7 ± 3.1 | 1.5 ± 0.6 | 1.9 ± 0.2 | 0.9 ± 0.1 | 0.2 ± 0.0 | 24.8 ± 0.6 | 17.5 ± 0.2 | 4.9 ± 2.1 | 1.7 ± 0.9 |
| Coculture | 52.4 ± 5.4 | 28.4 ± 3.9 | 36.0 ± 5.4 | 29.8 ± 4.0 | 0.6 ± 0.2 | 39.8 ± 4.1 | 38.4 ± 6.9 | 36.3 ± 3.8 | 49.4 ± 5.8 | 48.4 ± 1.5 | 28.3 ± 2.8 | 1.1 ± 0.4 | 0.4 ± 0.0 | 0.7 ± 0.5 | 35.3 ± 2.2 | 27.2 ± 3.3 | 9.6 ± 1.8 | 6.6 ± 2.5 |
| Student t test | P < .01 | P < .05 | NS | NS | NS | P < .01 | NS | NS | NS | NS | P < .001 | NS | NS | NS | P < .05 | P < .05 | P < .05 | P < .05 |
| hBM-MSCs | ||||||||||||||||||
| Alone | 0.7 ± 0.1 | 0.9 ± 0.0 | 1.2 ± 0.3 | 1.6 ± 1.7 | 0.3 ± 0.0 | 0.4 ± 0.0 | 31.8 ± 0.3 | 32.4 ± 0.5 | 7.1 ± 1.4 | 6.5 ± 2.3 | 7.2 ± 0.2 | 6.5 ± 0.7 | 0.7 ± 0.1 | 0.5 ± 0.1 | 30.6 ± 5.3 | 26.3 ± 1.7 | 1.6 ± 0.2 | 3.1 ± 0.7 |
| Coculture | 1.3 ± 0.4 | 1.6 ± 0.7 | 1.9 ± 0.5 | 1.1 ± 1.4 | 0.5 ± 0.0 | 0.3 ± 0.0 | 34.3 ± 0.1 | 35.6 ± 0.8 | 7.9 ± 1.9 | 8.6 ± 1.7 | 8.9 ± 0.7 | 8.1 ± 0.4 | 0.5 ± 0.0 | 0.4 ± 0.0 | 51.3 ± 2.2 | 37.2 ± 5.7 | 8.0 ± 4.1 | 9.1 ± 3.6 |
| Student t test | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | P < 0.01 | P < 0.05 | P < 0.05 | P < 0.05 |
Mean values ± SD of MFI (n = 10). The results were expressed as mean of geometric MFI values of the hBM-MSC (CD73+CD45−) or B-ALL cell (CD19+CD45+) population expressing Notch ligands and receptors.
B-ALL indicates B-lineage acute lymphoblastic leukemia; hBM-MSC, human bone marrow–mesenchymal stromal cell; DLL, Delta-like ligand; MFI, mean fluorescence intensity; and NS, nonsignificant.