Table 3 TTCF antibodies generated from... | American Society of Hematology

Table 3

TTCF antibodies generated from plasmablasts and memory B cells

B-cell subset	TTCF Ab ID (donor)	K_D, nM	Heavy chain						Kappa light chain
B-cell subset	TTCF Ab ID (donor)	K_D, nM	V-D-J junction	V	D	J	V-Mut nt/aa*	CDRs†	V-J junction	V	J	V-Mut nt/aa*	CDRs†
Plasmablast	Ab 1 (D1)	2.2	CVKREYYNDNSGYHVWGDYW	V3-23	D3-22	J5	32/18	8.8.18	CQEYDNWPRFTF	V3D-15	J3	14/7	6.3.10
	Ab 2 (D1)	0.32	CGRSVIGTVDNW	V3-11	D6-19	J4	34/18	8.8.10	CQQSKSWPQLTF	V3-15	J4	14/9	6.3.10
Memory	Ab 3 (D4)	0.38	CAKDTGSSELLLDNW	V3-30	D1-26	J4	23/14	8.8.13	CQQNSIWPPLTF	V3-11	J4	15/10	6.3.10
	Ab 4 (D2)	0.23	CARDPTTVTGNSLLDMW	V1-18	D4-17	J3	28/14	8.8.15	CQQSYSTPRVTF	V1-39 or V1D-39	J3	25/12	6.3.10
	Ab 5 (D1)	1.4	CATEPGLHCGPDCPDAFEIW	V4-31	D2-21	J3	20/13	10.7.18	CQHHHTF	V1-9	J2	17/10	6.3.5

B-cell subset	TTCF Ab ID (donor)	K_D, nM	Heavy chain						Kappa light chain
B-cell subset	TTCF Ab ID (donor)	K_D, nM	V-D-J junction	V	D	J	V-Mut nt/aa*	CDRs†	V-J junction	V	J	V-Mut nt/aa*	CDRs†
Plasmablast	Ab 1 (D1)	2.2	CVKREYYNDNSGYHVWGDYW	V3-23	D3-22	J5	32/18	8.8.18	CQEYDNWPRFTF	V3D-15	J3	14/7	6.3.10
	Ab 2 (D1)	0.32	CGRSVIGTVDNW	V3-11	D6-19	J4	34/18	8.8.10	CQQSKSWPQLTF	V3-15	J4	14/9	6.3.10
Memory	Ab 3 (D4)	0.38	CAKDTGSSELLLDNW	V3-30	D1-26	J4	23/14	8.8.13	CQQNSIWPPLTF	V3-11	J4	15/10	6.3.10
	Ab 4 (D2)	0.23	CARDPTTVTGNSLLDMW	V1-18	D4-17	J3	28/14	8.8.15	CQQSYSTPRVTF	V1-39 or V1D-39	J3	25/12	6.3.10
	Ab 5 (D1)	1.4	CATEPGLHCGPDCPDAFEIW	V4-31	D2-21	J3	20/13	10.7.18	CQHHHTF	V1-9	J2	17/10	6.3.5

CDR indicates complementary determining region.

Number of mutations detected in V-gene segment (nucleotides/amino acids).

CDR1, 2, and 3 lengths in amino acids.