BCR variable-domain analysis of tetramer-positive plasmablasts isolated from donor 1
| ID . | Day . | Heavy chain . | Kappa light chain . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| V-D-J junction . | V . | D . | J . | V-Mut nt/aa* . | CDRs† . | V-J junction . | V . | J . | V-Mut nt/aa* . | CDRs† . | ||
| PB01 | D6 | CARGRRYTFGYKSIPHDFW | V1-8 | D5-5 | J4 | 37/19 | 8.8.17 | CMQSSQIPHTF | V2-24 | J5 | 18/12 | 11.3.9 |
| PB02 | D7 | CARDRRQYSGHADLDYW | V1-18 | D5-12 | J4 | 11/5 | 8.8.15 | CQQTYSTLYTF | V1-39 | J2 | 14/4 | 6.3.9 |
| PB03 | D7 | CVRDSPYSSGSIPSAFDIW | V1-18 | D6-19 | J3 | 24/11 | 8.8.17 | CLQHFTYPYTF | V1-17 | J1 | 16/9 | 6.3.9 |
| PB04 | D7 | CARSVRGVVPFDYW | V2-5 | D3-10 | J4 | 18/13 | 10.7.12 | CQQYNTYSPWTF | V1-5 | J1 | 15/10 | 6.3.10 |
| PB05 | D6 | CSRDGGSRRGFAYW | V3-11 | D3-16 | J4 | 44/17 | 8.7.12 | CQHRSKWPPMVTF | V3-11 | J2 | 13/8 | 6.3.11 |
| PB06 | D6 | CGRSVIGTVDNW | V3-11 | D6-19 | J4 | 34/18 | 8.8.10 | CQQSKSWPQLTF | V3-15 | J4 | 14/9 | 6.3.10 |
| PB07 | D6 | CARATLPAAIGYYGMDVW | V3-21 | D2-2 | J6 | 10/4 | 8.8.16 | |||||
| PB08 | D6 | CAKDLQLWLLGAFDIR | V3-23 | D5-5 | J3 | 16/10 | 8.8.14 | CLQNCNCHRF | V1-6 | J1 | 24/18 | 6.3.8 |
| PB09‡ | D6 | CVKREYYNDNSGYHVWGDYW | V3-23 | D3-22 | J5 | 32/18 | 8.8.18 | CQEYDNWPRFTF | V3D-15 | J3 | 14/7 | 6.3.10 |
| PB10‡ | D7 | CVKREYYNDNSGYHVWGDYW | V3-23 | D3-22 | J5 | 33/21 | 8.8.18 | CQNYNNWPRFSF | V3-15 | J3 | 14/4 | 6.3.10 |
| PB11 | D7 | CARGQIWSNLPTLFEYW | V3-30 | D5-5 | J4 | 22/11 | 8.8.15 | CQHYNSYPLTF | V1-16 | J4 | 18/11 | 6.3.9 |
| PB12‡ | D6 | CARERGALLTRGHFDYW | V3-30-3 | D2-2 | J4 | 16/13 | 8.8.15 | CQHRSKWPPMVTF | V3-11 | J2 | 14/9 | 6.3.11 |
| PB13‡ | D7 | CARERGAMVTRGHFDYW | V3-30-3 | D5-5 | J4 | 25/16 | 8.8.15 | CQLRTTWPSMYTF | V3-11 | J2 | 22/12 | 6.3.11 |
| PB14‡ | D6 | CARDVFRVSGPLVAVGGFDYW | V3-30-3 | D6-19 | J4 | 29/12 | 8.8.19 | CQQYENWPPLTF | V3-15 | J4 | 12/9 | 6.3.10 |
| PB15‡ | D7 | CARDVFRVSGPLVAVGGFDYW | V3-30-3 | D6-19 | J4 | 31/12 | 8.8.19 | CQQYEKWPPLTF | V3-15 | J4 | 21/13 | 6.3.10 |
| PB16 | D7 | CARGLDHNSYVYYFDHW | V3-33 | D1-1 | J4 | 23/12 | 8.8.15 | CQQYNTWPPFTF | V3-15 | J3 | 12/7 | 6.3.10 |
| PB17 | D6 | CARDRDYSGSGIPPSFDPW | V3-33 | D3-10 | J5 | 17/11 | 8.8.17 | CQQSYIPPPAF | V1-39 | J4 | 17/12 | 6.3.9 |
| PB18 | D6 | CVREDIVLRVFAIFDYW | V3-74 | D2-8 | J4 | 25/12 | 8.8.15 | CQQSHTTPRTF | V1-39 or V1D-39 | J1 | 26/14 | 6.3.9 |
| PB19 | D7 | CARSGGSSSGSFYPGALYYNGMDVW | V4-4 | D3-10 | J6 | 16/9 | 8.7.23 | CQQSYSATWTF | V1-39 or V1D-39 | J1 | 18/9 | 6.3.9 |
| PB20 | D6 | CARAGPVGGSWHSVWFDLW | V4-31 | D2-15 | J5 | 24/13 | 10.7.17 | CQQRHNLITF | V3-11 | J5 | 16/7 | 6.3.8 |
| PB21 | D6 | CVRGYRFCSSSSCNEWYFDLW | V4-31 | D2-2 | J2 | 30/17 | 10.7.19 | CHQSRSLPHTF | V6-21 or V6D-21 | J2 | 8/5 | 6.3.9 |
| PB22 | D7 | CATEPGLHCGPDCPDAFEIW | V4-31 | D2-21 | J3 | 25/14 | 10.7.18 | CQHHHTF | V1-9 | J2 | 18/11 | 6.3.5 |
| PB23‡ | D6 | CAREFINTLGGVVDHYGIDVW | V4-31 | D3-16 | J6 | 13/8 | 10.7.19 | |||||
| PB24‡ | D6 | CARGFMNTLGGVIDTEGLDVW | V4-31 | D3-16 | J6 | 42/23 | 10.7.19 | |||||
| PB25 | D6 | CARVTTHYDPPFDHW | V4-31 | D4-4 | J4 | 39/19 | 10.7.13 | CQQYYSTPWTF | V4-1 | J1 | 10/4 | 12.3.9 |
| PB26 | D6 | CARNVVITLGGVIESNYYFDYW | V4-39 | D3-16 | J4 | 23/13 | 10.7.20 | CMQGIQTPTF | V2-28 | J1 | 11/4 | 11.3.8 |
| PB27‡ | D7 | CGLMADNWFDPW | V4-39 | D2-8 | J5 | 28/13 | 10.7.10 | CQQYNNWPPITF | V3-15 | J5 | 2/1 | 6.3.10 |
| PB28‡ | D7 | CALQTDNWFDPW | V4-39 | D4-4 | J5 | 34/17 | 10.7.10 | CQQYYQWPPYTF | V3D-15 | J2 | 22/10 | 6.3.10 |
| PB29‡ | D7 | CARQADNWCDPW | V4-39 | D6-19 | J5 | 31/16 | 10.7.10 | CQQYYNWPPYTF | V3D-15 | J2 | 9/8 | 6.3.10 |
| PB30 | D7 | CARGWFGYLDYW | V4-59 | D3-10 | J4 | 13/8 | 8.7.10 | |||||
| PB31 | D7 | CARYCSSPTCSVVYGMDVW | V5-51 | D2-2 | J6 | 15/8 | 8.8.17 | CQQTNSFPFNF | V1D-12 | J4 | 27/13 | 6.3.9 |
| PB32 | D6 | CWSSVYQSDGGGYYYVDYW | V5-51 | D3-22 | J4 | 31/14 | 8.8.17 | CQETYTTPPLTF | V1-39 | J4 | 24/11 | 6.3.10 |
| PB33 | D6 | CQHRSKWPPMVTF | V3-11 | J2 | 13/8 | 6.3.11 | ||||||
| PB34 | D6 | CQQYSSYPLTF | V1-16 | J4 | 19/10 | 6.3.9 | ||||||
| ID . | Day . | Heavy chain . | Kappa light chain . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| V-D-J junction . | V . | D . | J . | V-Mut nt/aa* . | CDRs† . | V-J junction . | V . | J . | V-Mut nt/aa* . | CDRs† . | ||
| PB01 | D6 | CARGRRYTFGYKSIPHDFW | V1-8 | D5-5 | J4 | 37/19 | 8.8.17 | CMQSSQIPHTF | V2-24 | J5 | 18/12 | 11.3.9 |
| PB02 | D7 | CARDRRQYSGHADLDYW | V1-18 | D5-12 | J4 | 11/5 | 8.8.15 | CQQTYSTLYTF | V1-39 | J2 | 14/4 | 6.3.9 |
| PB03 | D7 | CVRDSPYSSGSIPSAFDIW | V1-18 | D6-19 | J3 | 24/11 | 8.8.17 | CLQHFTYPYTF | V1-17 | J1 | 16/9 | 6.3.9 |
| PB04 | D7 | CARSVRGVVPFDYW | V2-5 | D3-10 | J4 | 18/13 | 10.7.12 | CQQYNTYSPWTF | V1-5 | J1 | 15/10 | 6.3.10 |
| PB05 | D6 | CSRDGGSRRGFAYW | V3-11 | D3-16 | J4 | 44/17 | 8.7.12 | CQHRSKWPPMVTF | V3-11 | J2 | 13/8 | 6.3.11 |
| PB06 | D6 | CGRSVIGTVDNW | V3-11 | D6-19 | J4 | 34/18 | 8.8.10 | CQQSKSWPQLTF | V3-15 | J4 | 14/9 | 6.3.10 |
| PB07 | D6 | CARATLPAAIGYYGMDVW | V3-21 | D2-2 | J6 | 10/4 | 8.8.16 | |||||
| PB08 | D6 | CAKDLQLWLLGAFDIR | V3-23 | D5-5 | J3 | 16/10 | 8.8.14 | CLQNCNCHRF | V1-6 | J1 | 24/18 | 6.3.8 |
| PB09‡ | D6 | CVKREYYNDNSGYHVWGDYW | V3-23 | D3-22 | J5 | 32/18 | 8.8.18 | CQEYDNWPRFTF | V3D-15 | J3 | 14/7 | 6.3.10 |
| PB10‡ | D7 | CVKREYYNDNSGYHVWGDYW | V3-23 | D3-22 | J5 | 33/21 | 8.8.18 | CQNYNNWPRFSF | V3-15 | J3 | 14/4 | 6.3.10 |
| PB11 | D7 | CARGQIWSNLPTLFEYW | V3-30 | D5-5 | J4 | 22/11 | 8.8.15 | CQHYNSYPLTF | V1-16 | J4 | 18/11 | 6.3.9 |
| PB12‡ | D6 | CARERGALLTRGHFDYW | V3-30-3 | D2-2 | J4 | 16/13 | 8.8.15 | CQHRSKWPPMVTF | V3-11 | J2 | 14/9 | 6.3.11 |
| PB13‡ | D7 | CARERGAMVTRGHFDYW | V3-30-3 | D5-5 | J4 | 25/16 | 8.8.15 | CQLRTTWPSMYTF | V3-11 | J2 | 22/12 | 6.3.11 |
| PB14‡ | D6 | CARDVFRVSGPLVAVGGFDYW | V3-30-3 | D6-19 | J4 | 29/12 | 8.8.19 | CQQYENWPPLTF | V3-15 | J4 | 12/9 | 6.3.10 |
| PB15‡ | D7 | CARDVFRVSGPLVAVGGFDYW | V3-30-3 | D6-19 | J4 | 31/12 | 8.8.19 | CQQYEKWPPLTF | V3-15 | J4 | 21/13 | 6.3.10 |
| PB16 | D7 | CARGLDHNSYVYYFDHW | V3-33 | D1-1 | J4 | 23/12 | 8.8.15 | CQQYNTWPPFTF | V3-15 | J3 | 12/7 | 6.3.10 |
| PB17 | D6 | CARDRDYSGSGIPPSFDPW | V3-33 | D3-10 | J5 | 17/11 | 8.8.17 | CQQSYIPPPAF | V1-39 | J4 | 17/12 | 6.3.9 |
| PB18 | D6 | CVREDIVLRVFAIFDYW | V3-74 | D2-8 | J4 | 25/12 | 8.8.15 | CQQSHTTPRTF | V1-39 or V1D-39 | J1 | 26/14 | 6.3.9 |
| PB19 | D7 | CARSGGSSSGSFYPGALYYNGMDVW | V4-4 | D3-10 | J6 | 16/9 | 8.7.23 | CQQSYSATWTF | V1-39 or V1D-39 | J1 | 18/9 | 6.3.9 |
| PB20 | D6 | CARAGPVGGSWHSVWFDLW | V4-31 | D2-15 | J5 | 24/13 | 10.7.17 | CQQRHNLITF | V3-11 | J5 | 16/7 | 6.3.8 |
| PB21 | D6 | CVRGYRFCSSSSCNEWYFDLW | V4-31 | D2-2 | J2 | 30/17 | 10.7.19 | CHQSRSLPHTF | V6-21 or V6D-21 | J2 | 8/5 | 6.3.9 |
| PB22 | D7 | CATEPGLHCGPDCPDAFEIW | V4-31 | D2-21 | J3 | 25/14 | 10.7.18 | CQHHHTF | V1-9 | J2 | 18/11 | 6.3.5 |
| PB23‡ | D6 | CAREFINTLGGVVDHYGIDVW | V4-31 | D3-16 | J6 | 13/8 | 10.7.19 | |||||
| PB24‡ | D6 | CARGFMNTLGGVIDTEGLDVW | V4-31 | D3-16 | J6 | 42/23 | 10.7.19 | |||||
| PB25 | D6 | CARVTTHYDPPFDHW | V4-31 | D4-4 | J4 | 39/19 | 10.7.13 | CQQYYSTPWTF | V4-1 | J1 | 10/4 | 12.3.9 |
| PB26 | D6 | CARNVVITLGGVIESNYYFDYW | V4-39 | D3-16 | J4 | 23/13 | 10.7.20 | CMQGIQTPTF | V2-28 | J1 | 11/4 | 11.3.8 |
| PB27‡ | D7 | CGLMADNWFDPW | V4-39 | D2-8 | J5 | 28/13 | 10.7.10 | CQQYNNWPPITF | V3-15 | J5 | 2/1 | 6.3.10 |
| PB28‡ | D7 | CALQTDNWFDPW | V4-39 | D4-4 | J5 | 34/17 | 10.7.10 | CQQYYQWPPYTF | V3D-15 | J2 | 22/10 | 6.3.10 |
| PB29‡ | D7 | CARQADNWCDPW | V4-39 | D6-19 | J5 | 31/16 | 10.7.10 | CQQYYNWPPYTF | V3D-15 | J2 | 9/8 | 6.3.10 |
| PB30 | D7 | CARGWFGYLDYW | V4-59 | D3-10 | J4 | 13/8 | 8.7.10 | |||||
| PB31 | D7 | CARYCSSPTCSVVYGMDVW | V5-51 | D2-2 | J6 | 15/8 | 8.8.17 | CQQTNSFPFNF | V1D-12 | J4 | 27/13 | 6.3.9 |
| PB32 | D6 | CWSSVYQSDGGGYYYVDYW | V5-51 | D3-22 | J4 | 31/14 | 8.8.17 | CQETYTTPPLTF | V1-39 | J4 | 24/11 | 6.3.10 |
| PB33 | D6 | CQHRSKWPPMVTF | V3-11 | J2 | 13/8 | 6.3.11 | ||||||
| PB34 | D6 | CQQYSSYPLTF | V1-16 | J4 | 19/10 | 6.3.9 | ||||||
Bolded sequence pairs represent clonally related B cells.
CDR indicates complementarity determining region.
Number of mutations detected in V-gene segment (nucleotides/amino acids).
CDR1, 2, and 3 lengths in amino acids.
Five sets of related sequences were observed (4 pairs and one cluster composed of 3 related clones) that shared use of the same gene segments and a subset of mutations.