Table 2 B-cell populations with deletion... | American Society of Hematology

Table 2

B-cell populations with deletion efficiencies in Notch2IC//CD19Cre^+/+ and CD19Cre^+/+ control mice

Gated on:	BM (n = 4)				Spleen (n = 4)
Gated on:	IgM⁻ B220^low CD43⁺ pro-B	IgM⁻ B220^low CD43⁻ pre-B	B220⁺ IgM⁺ IgD⁻ immature B	B220⁺ IgM⁺ IgD⁺ mature B	AA4.1⁺ IgM⁺ T B	AA4.1⁺ IgM^high CD23⁻ T1 B	AA4.1⁺ IgM^high CD23⁺ T2 B	AA4.1⁺ IgM^low CD23⁺ T3 B	B220⁺ CD21^int CD23⁺ Fo B	B220⁺ CD21^high CD23⁻ MZ B
CD19Cre^+/+, mean ± SD. %	10 ± 2	43 ± 14	16 ± 4	20 ± 4	27 ± 5	21 ± 0	49 ± 1	12 ± 2	78 ± 2	2 ± 1
Notch2IC//CD19Cre^+/+, mean ± SD. %	9 ± 2	21 ± 12*	13 ± 1	3 ± 1‡	17 ± 5	38 ± 4‡	25 ± 8†	11 ± 2†	8 ± 3‡	71 ± 10‡
CD19Cre^+/+, mean ± SD, × 10⁵	1.9 ± 0.5	7.9 ± 3.5	3.0 ± 1.6	3.4 ± 0.1	3.3 ± 1.9	0.9 ± 0.5	2.1 ± 1.1	0.5 ± 0.2	12.8 ± 5.4	0.4 ± 0.4
Notch2IC//CD19Cre^+/+, mean ± SD, × 10⁵	2.1 ± 1.3	3.1 ± 1.8	1.2 ± 0.3	0.3 ± 0.1†	1.7 ± 0.9	1.0 ± 0.1	0.6 ± 0.1‡	0.3 ± 0.1†	0.6 ± 0.2‡	7.0 ± 1.1‡
Notch2IC//CD19Cre^+/+, mean ± SD, % hCD2⁺	17 ± 11	33 ± 3	70 ± 2	74 ± 10	69 ± 9	94 ± 3	88 ± 8	68 ± 7	48 ± 15	94 ± 5

Gated on:	BM (n = 4)				Spleen (n = 4)
Gated on:	IgM⁻ B220^low CD43⁺ pro-B	IgM⁻ B220^low CD43⁻ pre-B	B220⁺ IgM⁺ IgD⁻ immature B	B220⁺ IgM⁺ IgD⁺ mature B	AA4.1⁺ IgM⁺ T B	AA4.1⁺ IgM^high CD23⁻ T1 B	AA4.1⁺ IgM^high CD23⁺ T2 B	AA4.1⁺ IgM^low CD23⁺ T3 B	B220⁺ CD21^int CD23⁺ Fo B	B220⁺ CD21^high CD23⁻ MZ B
CD19Cre^+/+, mean ± SD. %	10 ± 2	43 ± 14	16 ± 4	20 ± 4	27 ± 5	21 ± 0	49 ± 1	12 ± 2	78 ± 2	2 ± 1
Notch2IC//CD19Cre^+/+, mean ± SD. %	9 ± 2	21 ± 12*	13 ± 1	3 ± 1‡	17 ± 5	38 ± 4‡	25 ± 8†	11 ± 2†	8 ± 3‡	71 ± 10‡
CD19Cre^+/+, mean ± SD, × 10⁵	1.9 ± 0.5	7.9 ± 3.5	3.0 ± 1.6	3.4 ± 0.1	3.3 ± 1.9	0.9 ± 0.5	2.1 ± 1.1	0.5 ± 0.2	12.8 ± 5.4	0.4 ± 0.4
Notch2IC//CD19Cre^+/+, mean ± SD, × 10⁵	2.1 ± 1.3	3.1 ± 1.8	1.2 ± 0.3	0.3 ± 0.1†	1.7 ± 0.9	1.0 ± 0.1	0.6 ± 0.1‡	0.3 ± 0.1†	0.6 ± 0.2‡	7.0 ± 1.1‡
Notch2IC//CD19Cre^+/+, mean ± SD, % hCD2⁺	17 ± 11	33 ± 3	70 ± 2	74 ± 10	69 ± 9	94 ± 3	88 ± 8	68 ± 7	48 ± 15	94 ± 5

BM cells were isolated from one tibia bone per mouse. Percentages within the lymphocyte gate are shown.

P <.05 in comparison to control.

P < .01 in comparison to control.

P < .001 in comparison to control.