Summary of VH186.2 sequences from NP-specific IgG1 B cells
| . | Wild-type . | Puma−/− . |
|---|---|---|
| Single cell PCR, no. | 24 | 53 |
| Positive PCR, no. | 17 | 28 |
| Positive VH186.2, no. | 14 | 19 |
| Range mutations per VH186.2, no. | 0-8 | 0-12 |
| Sequences with zero mutation, % | 7.1 | 10.5 |
| Average total mutations per VH186.2, no. (SD) | 4.9 ± 2.3 | 6.6 ± 3.4 |
| W33L substitution, n/N (%) | 6/14 (42.9) | 7/19 (36.8) |
| CDR1+CDR2, R/S (ratio) | 37/5 (7.4) | 51/17 (3.0) |
| FW1-FW3, R/S (ratio) | 16/10 (1.6) | 33/20 (1.7) |
| . | Wild-type . | Puma−/− . |
|---|---|---|
| Single cell PCR, no. | 24 | 53 |
| Positive PCR, no. | 17 | 28 |
| Positive VH186.2, no. | 14 | 19 |
| Range mutations per VH186.2, no. | 0-8 | 0-12 |
| Sequences with zero mutation, % | 7.1 | 10.5 |
| Average total mutations per VH186.2, no. (SD) | 4.9 ± 2.3 | 6.6 ± 3.4 |
| W33L substitution, n/N (%) | 6/14 (42.9) | 7/19 (36.8) |
| CDR1+CDR2, R/S (ratio) | 37/5 (7.4) | 51/17 (3.0) |
| FW1-FW3, R/S (ratio) | 16/10 (1.6) | 33/20 (1.7) |
Details of the nucleotide sequences of IgVH186.2 genes are summarized, comparing wild-type with Puma−/− B cells.
R/S indicates ratio of replacement to silent mutations in all sequences; CDR, complementary-determining region; and FW, framework region.