Table 2 Characteristics of mAb 37.1 and... | American Society of Hematology

Table 2

Characteristics of mAb 37.1 and mAb 37_surr

Function	Cell type (human/cynomolgus)	mAb 37.1	mAb 37_surr
Apparent affinity, K_D	B cells/B cells	0.4nM	0.2nM
Apparent affinity, K_D	T cells/T cells	1.7nM	1.4nM
Ag density, sites/cell	B cells/B cells	53 000	55 000
Ag density, sites/cell	T cells/T cells	1900	1900
ADCC (EC₅₀)	Ramos/macCD37-CHO	0.01nM (2 ng/mL)	0.02nM (4 ng/mL)
Apoptosis, without x-link (EC₅₀)	Ramos/macCD37-CHO	0.4nM (66 ng/mL)	9.3nM (1423 ng/mL)
Apoptosis, with x-link (EC₅₀)	Ramos/macCD37-CHO	0.3nM (41 ng/mL)	10nM (1574 ng/mL)
CDC	Ramos/macCD37-CHO	Negative	Negative
Whole blood (EC₅₀)	B cells/B cells	0.2nM (33 ng/mL)	0.9nM (140 ng/mL)

Function	Cell type (human/cynomolgus)	mAb 37.1	mAb 37_surr
Apparent affinity, K_D	B cells/B cells	0.4nM	0.2nM
Apparent affinity, K_D	T cells/T cells	1.7nM	1.4nM
Ag density, sites/cell	B cells/B cells	53 000	55 000
Ag density, sites/cell	T cells/T cells	1900	1900
ADCC (EC₅₀)	Ramos/macCD37-CHO	0.01nM (2 ng/mL)	0.02nM (4 ng/mL)
Apoptosis, without x-link (EC₅₀)	Ramos/macCD37-CHO	0.4nM (66 ng/mL)	9.3nM (1423 ng/mL)
Apoptosis, with x-link (EC₅₀)	Ramos/macCD37-CHO	0.3nM (41 ng/mL)	10nM (1574 ng/mL)
CDC	Ramos/macCD37-CHO	Negative	Negative
Whole blood (EC₅₀)	B cells/B cells	0.2nM (33 ng/mL)	0.9nM (140 ng/mL)

ADCC indicates Ab-dependent cell-mediated cytotoxicity; and CDC, complement-dependent cytotoxicity.