CD4+ RTEs remain flexible in their Th responses
| Day . | Th . | RTE:MN ratio . | Mean ratio of RTE to MN IFN-γ+ cells . | P . | Mean ratio of RTE to MN IL-4+ cells . | P . |
|---|---|---|---|---|---|---|
| D+3 | Th0 | RTE/MN only | 0.4 | .006* | ||
| 1:1 | 0.6 | .04* | ||||
| 1:5 | 0.5 | .018* | ||||
| D+5 | Th1 | RTE/MN only | 0.7 | .04* | ||
| 1:1 | 1.0 | .72 | ||||
| 1:5 | 1.0 | > .999 | ||||
| D+5 | Th2 | RTE/MN only | 1.6 | .002* | ||
| 1:1 | 1.5 | .003* | ||||
| 1:5 | 1.6 | .017* |
| Day . | Th . | RTE:MN ratio . | Mean ratio of RTE to MN IFN-γ+ cells . | P . | Mean ratio of RTE to MN IL-4+ cells . | P . |
|---|---|---|---|---|---|---|
| D+3 | Th0 | RTE/MN only | 0.4 | .006* | ||
| 1:1 | 0.6 | .04* | ||||
| 1:5 | 0.5 | .018* | ||||
| D+5 | Th1 | RTE/MN only | 0.7 | .04* | ||
| 1:1 | 1.0 | .72 | ||||
| 1:5 | 1.0 | > .999 | ||||
| D+5 | Th2 | RTE/MN only | 1.6 | .002* | ||
| 1:1 | 1.5 | .003* | ||||
| 1:5 | 1.6 | .017* |
Purified congenically marked CD4+ RTEs and MN T cells were cocultured at various ratios with antigen-presenting cells under Th0, Th1, and Th2 conditions for 3 (D +3) or 5 days (D +5). Data are presented as the mean ratio of IFN-γ or IL-4 production by RTEs to that by MN T cells (eg, % IFN-γ+ RTEs divided by % IFN-γ+ MN T cells). Data are compiled from 3 independent experiments with cells pooled from 2-3 mice per experiment.
Significant differences between populations calculated with an unpaired, 2-tailed Student t test.