XG-19 and XG-20 HMCLs were cultured at 105 cells/mL without cytokine or with IL-6 (2 ng/mL), or with 104 osteoclasts with or without anti-IL-6 (BE8) mAb (10 mg/mL), TACI-Fc (20 mg/mL), anti-IGF-1R mAb (4 mg/mL), anti-IL-10 mAb (10 mg/mL), or anti-CCR2 mAb (10 mg/mL)
| . | XG-20 . | XG-19 . | ||
|---|---|---|---|---|
| Annexin V, % . | P (N = 5) . | Annexin V, % . | P (N = 5) . | |
| No cytokine | 48 ± 9 | 90 ± 3 | ||
| IL-6 | 21 ± 7 | .008 (vs No cytokine) | 56 ± 4 | .0003 (vs No cytokine) |
| Osteoclasts | 16 ± 3 | .01 (vs No cytokine) | 41 ± 9 | .01 (vs No cytokine) |
| Osteoclasts + B-E8 | 25 ± 2 | .01 (vs Osteoclasts) | 51 ± 8 | .05 (vs Osteoclasts) |
| Osteoclasts + TACI-Fc | 29 ± 5 | .02 (vs Osteoclasts) | 67 ± 8 | .01 (vs Osteoclasts) |
| Osteoclasts + anti-IGF1R | 84 ± 8 | .0005 (vs Osteoclasts) | 76 ± 9 | .003 (vs Osteoclasts) |
| Osteoclasts + anti-IL-10 | 18 ± 2 | NS (vs Osteoclasts) | 42 ± 4 | NS (vs Osteoclasts) |
| Osteoclasts + anti-CCR2 | 19 ± 2 | NS (vs Osteoclasts) | 43 ± 3 | NS (vs Osteoclasts) |
| . | XG-20 . | XG-19 . | ||
|---|---|---|---|---|
| Annexin V, % . | P (N = 5) . | Annexin V, % . | P (N = 5) . | |
| No cytokine | 48 ± 9 | 90 ± 3 | ||
| IL-6 | 21 ± 7 | .008 (vs No cytokine) | 56 ± 4 | .0003 (vs No cytokine) |
| Osteoclasts | 16 ± 3 | .01 (vs No cytokine) | 41 ± 9 | .01 (vs No cytokine) |
| Osteoclasts + B-E8 | 25 ± 2 | .01 (vs Osteoclasts) | 51 ± 8 | .05 (vs Osteoclasts) |
| Osteoclasts + TACI-Fc | 29 ± 5 | .02 (vs Osteoclasts) | 67 ± 8 | .01 (vs Osteoclasts) |
| Osteoclasts + anti-IGF1R | 84 ± 8 | .0005 (vs Osteoclasts) | 76 ± 9 | .003 (vs Osteoclasts) |
| Osteoclasts + anti-IL-10 | 18 ± 2 | NS (vs Osteoclasts) | 42 ± 4 | NS (vs Osteoclasts) |
| Osteoclasts + anti-CCR2 | 19 ± 2 | NS (vs Osteoclasts) | 43 ± 3 | NS (vs Osteoclasts) |
Cells were recovered after 3 days of culture, and apoptotic cells were detected by annexin V staining. Results are the means ± SD of 5 experiments.
NS indicates not significantly different.