Table 1 A comparison of phenotypes... | American Society of Hematology

Table 1

A comparison of phenotypes observed in mTOR compromised mice (neo-containing mTOR KI; reduced mTOR protein levels) and neo-less mTOR KI mice (KI^neo−; normal mTOR protein levels) relative to phenotypes seen in WT mice*

Phenotype	mTOR KI	mTOR KI^neo−
Body size	Reduced by 30%	Normal
Organ sizes	Spleens much smaller	Spleens normal
Cell sizes	Reduced	Normal
mTOR protein levels	Reduced by 70%	Normal
RAPTOR protein levels	Decreased	Normal
RICTOR protein levels	Decreased	Normal
DEPTOR protein levels	Slightly increased	Normal
mTORC1 target pS6K	Decreased	Normal
mTORC1 target p4EBP1†	Decreased (BM)	Decreased (BM)
mTORC2 target pAKTSer473	Decreased	Normal
mTORC1 target pS6K activated T cells	Decreased	Normal
mTORC1 target pS6K activated B cells	Decreased	Normal
mTORC1 target pS6K insulin-stimulate MEFs	Decreased	Not tested
mTORC2 target pAKT activated T cells	Decreased	Normal
mTORC2 target pAKT activated B cells	Increased	Normal
mTORC2 target pAKT insulin-stimulated MEFs	Decreased	Not tested
Thymic T cells	Reduced	Normal
Splenic T cells	Reduced/fewer memory	Normal
LN T cells	Reduced	Normal
Proliferation rates of splenic T cells	Reduced/defective	Normal
BM B cells	Reduced/partial block	Normal
Splenic B cells	Reduced/fewer T1, MZ, FO	Normal
LN B cells	Reduced	Normal
Proliferation/survival splenic B cells	Reduced/defective	Normal
Antibody production	Reduced	Normal
BM Gr1⁺ cells	Normal	Not tested
Splenic Gr1⁺ cells	Decreased	Not tested
BM Mac-1⁺ cells	Normal	Not tested
Splenic Mac-1⁺ cells	Decreased	Not tested
T-cell migration in spleen, SDF, TECK	Decreased	Not tested
T-cell migration in spleen, MIP3B	Normal	Not tested
B-cell migration in spleen, SDF, S1P	Decreased	Not tested
Neutrophil migration in BM, fMLP	Normal	Not tested

Phenotype	mTOR KI	mTOR KI^neo−
Body size	Reduced by 30%	Normal
Organ sizes	Spleens much smaller	Spleens normal
Cell sizes	Reduced	Normal
mTOR protein levels	Reduced by 70%	Normal
RAPTOR protein levels	Decreased	Normal
RICTOR protein levels	Decreased	Normal
DEPTOR protein levels	Slightly increased	Normal
mTORC1 target pS6K	Decreased	Normal
mTORC1 target p4EBP1†	Decreased (BM)	Decreased (BM)
mTORC2 target pAKTSer473	Decreased	Normal
mTORC1 target pS6K activated T cells	Decreased	Normal
mTORC1 target pS6K activated B cells	Decreased	Normal
mTORC1 target pS6K insulin-stimulate MEFs	Decreased	Not tested
mTORC2 target pAKT activated T cells	Decreased	Normal
mTORC2 target pAKT activated B cells	Increased	Normal
mTORC2 target pAKT insulin-stimulated MEFs	Decreased	Not tested
Thymic T cells	Reduced	Normal
Splenic T cells	Reduced/fewer memory	Normal
LN T cells	Reduced	Normal
Proliferation rates of splenic T cells	Reduced/defective	Normal
BM B cells	Reduced/partial block	Normal
Splenic B cells	Reduced/fewer T1, MZ, FO	Normal
LN B cells	Reduced	Normal
Proliferation/survival splenic B cells	Reduced/defective	Normal
Antibody production	Reduced	Normal
BM Gr1⁺ cells	Normal	Not tested
Splenic Gr1⁺ cells	Decreased	Not tested
BM Mac-1⁺ cells	Normal	Not tested
Splenic Mac-1⁺ cells	Decreased	Not tested
T-cell migration in spleen, SDF, TECK	Decreased	Not tested
T-cell migration in spleen, MIP3B	Normal	Not tested
B-cell migration in spleen, SDF, S1P	Decreased	Not tested
Neutrophil migration in BM, fMLP	Normal	Not tested

See supplemental Figure 11.

†

The mTORC1 downstream target 4EBP1 showed decreased phosphorylation in the BM of KI^neo− mice and represents the only observed phenotype attributable to the 628C allele.

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