In vivo phagocytosis of activated platelets by leukocytes: role of P-selectin
| . | . | Platelet-neutrophil heterotypic aggregates, % 7/4+ cells . | Neutrophils with intracellular platelets, % 7/4+ cells . | Platetelet-monocyte heterotypic aggregates, % CD14+ cells . | Monocytes with intracellular platelets, % CD14+ cells . |
|---|---|---|---|---|---|
| Untreated | 1.1 ± 0.1 | 1.0 ± 0.8 | 1.7 ± 0.9 | 0.9 ± 0.3 | |
| 1 h | Resting WT | 3.7 ± 1.2 | 2.0 ± 0.8 | 2.9 ± 1.1 | 1.1 ± 0.7 |
| Activated WT | 21.0 ± 4.0† | 73.0 ± 5.9† | 62.0 ± 9.2† | 2.0 ± 2.6 | |
| Activated P-sel−/− | 7.5 ± 2.4* | 5.3 ± 2.9* | 7.7 ± 2.1† | 9.0 ± 1.0† | |
| 3 h | Resting WT | 3.1 ± 0.8 | 4.1 ± 0.9 | 2.2 ± 0.8 | 1.6 ± 0.3 |
| Activated WT | 16.0 ± 1.7† | 23.0 ± 2.0† | 47.3 ± 11.0† | 17.7 ± 8.3† | |
| Activated P-sel−/− | 8.5 ± 2.4*† | 7.3 ± 4.5*† | 9.0 ± 2.6* | 12.7 ± 5.9 † | |
| 24 h | Resting WT | 2.5 ± 1.8 | 6.3 ± 1.5 | 3.1 ± 1.3 | 1.0 ± 0.9 |
| Activated WT | 5.1 ± 0.3 | 6.3 ± 1.5 | 38.3 ± 12.5† | 66.0 ± 7.9† | |
| Activated P-sel−/− | 7.3 ± 1.0* | 43.5 ± 3.7*† | 7.0 ± 1.0* | 46.5 ± 4.5† |
| . | . | Platelet-neutrophil heterotypic aggregates, % 7/4+ cells . | Neutrophils with intracellular platelets, % 7/4+ cells . | Platetelet-monocyte heterotypic aggregates, % CD14+ cells . | Monocytes with intracellular platelets, % CD14+ cells . |
|---|---|---|---|---|---|
| Untreated | 1.1 ± 0.1 | 1.0 ± 0.8 | 1.7 ± 0.9 | 0.9 ± 0.3 | |
| 1 h | Resting WT | 3.7 ± 1.2 | 2.0 ± 0.8 | 2.9 ± 1.1 | 1.1 ± 0.7 |
| Activated WT | 21.0 ± 4.0† | 73.0 ± 5.9† | 62.0 ± 9.2† | 2.0 ± 2.6 | |
| Activated P-sel−/− | 7.5 ± 2.4* | 5.3 ± 2.9* | 7.7 ± 2.1† | 9.0 ± 1.0† | |
| 3 h | Resting WT | 3.1 ± 0.8 | 4.1 ± 0.9 | 2.2 ± 0.8 | 1.6 ± 0.3 |
| Activated WT | 16.0 ± 1.7† | 23.0 ± 2.0† | 47.3 ± 11.0† | 17.7 ± 8.3† | |
| Activated P-sel−/− | 8.5 ± 2.4*† | 7.3 ± 4.5*† | 9.0 ± 2.6* | 12.7 ± 5.9 † | |
| 24 h | Resting WT | 2.5 ± 1.8 | 6.3 ± 1.5 | 3.1 ± 1.3 | 1.0 ± 0.9 |
| Activated WT | 5.1 ± 0.3 | 6.3 ± 1.5 | 38.3 ± 12.5† | 66.0 ± 7.9† | |
| Activated P-sel−/− | 7.3 ± 1.0* | 43.5 ± 3.7*† | 7.0 ± 1.0* | 46.5 ± 4.5† |
Fluorescent platelets from wild type (WT) or P-sel−/− mice were activated (or not) and injected in the tail vein of WT mice. Leukocytes with intracellular fluorescent platelets were identified by flow cytometry at different times after injection; see text for experimental details. Results are expressed as mean ± SEM.
Statistically different from mice injected with activated WT platelets, P < .05.
Statistically different from mice injected with resting platelets, P < .05.