Table 1 A tabular representation of... | American Society of Hematology

Table 1

A tabular representation of IRS-2 inhibition characteristics for the targeted serine proteases

Enzyme	Amount of enzyme used, nM	IRS2 IC₅₀, nM	IC₅₀/enzyme ratio	Inhibitor characteristics
Cathepsin G	12.2	11.5 ± 0.7	0.94	Fast binding tight
Chymase	3.6	4 ± 0.2	1.11	Slow binding tight
Thrombin	0.02	170.3 ± 11	8515	Slow binding classical
α-chymotrypsin	0.038	0.38 ± 0.02	10	N/A
Trypsin	0.25	562.7 ± 27.3	2251	N/A

Enzyme	Amount of enzyme used, nM	IRS2 IC₅₀, nM	IC₅₀/enzyme ratio	Inhibitor characteristics
Cathepsin G	12.2	11.5 ± 0.7	0.94	Fast binding tight
Chymase	3.6	4 ± 0.2	1.11	Slow binding tight
Thrombin	0.02	170.3 ± 11	8515	Slow binding classical
α-chymotrypsin	0.038	0.38 ± 0.02	10	N/A
Trypsin	0.25	562.7 ± 27.3	2251	N/A

The amount of enzyme used in the assays as well as the inhibitory concentration at half-maximum (IC₅₀) of IRS-2 for these enzymes is stated in the first and second columns. The third column represents the ratio between IRS-2 IC₅₀ and enzyme concentration, and the last column shows the binding characteristics of the inhibitor to the targeted enzymes with the relevance in host physiology (ie, tight or classical inhibitor, fast binding, or slow binding to the enzyme). Because trypsin and α-chymotrypsin were not physiologically relevant to our studies, we did not further study their inhibition characteristics, as depicted in by N/A.

NA indicates not applicable.

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