Evidence of phenotypic maturation of pDCs produced on OP9-Del1
. | Mean fluorescence intensity of CD86 on BDCA-2+ cells . | . | . | ||
|---|---|---|---|---|---|
| Stimulus . | None . | CpG . | CD40L + IL-1 . | ||
| Exp. 1 | 169 | 428 | 741 | ||
| Exp. 2 | 95 | 535 | 442 | ||
| Exp. 3 | 88 | 138 | 220 | ||
| Exp. 4 | 219 | 562 | N.D. | ||
. | Mean fluorescence intensity of CD86 on BDCA-2+ cells . | . | . | ||
|---|---|---|---|---|---|
| Stimulus . | None . | CpG . | CD40L + IL-1 . | ||
| Exp. 1 | 169 | 428 | 741 | ||
| Exp. 2 | 95 | 535 | 442 | ||
| Exp. 3 | 88 | 138 | 220 | ||
| Exp. 4 | 219 | 562 | N.D. | ||
Cells produced on OP9-Del1 stroma after 20 days were stimulated for 72 hours with the indicated agents, and the expression levels of CD86 were measured by multicolor flow cytometry after gating on BDCA-2+ cells.
Exp. indicates experiment; N.D., not done.