Cytokine production induced by various stimuli
. | None . | . | CpG . | . | CD40L + IL-1 . | . | PGN . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | Del1 . | Control . | Del1 . | Control . | Del1 . | Control . | Del1 . | Control . | ||||
| IFN-α, pg/mL | ||||||||||||
| Exp. 1 | ND | ND | 15 238 | <10 | <10 | <10 | <10 | <10 | ||||
| Exp. 2 | ND | ND | 18 163 | 8 463 | <10 | <10 | <10 | <10 | ||||
| Exp. 3 | <10 | <10 | 20 475 | 60 | <10 | <10 | 50 | ND | ||||
| Exp. 4 | 55 | <10 | 10 655 | 7 700 | <10 | <10 | ND | ND | ||||
| Exp. 5 | <10 | 85 | 11 450 | 12 805 | <10 | <10 | ND | ND | ||||
| Exp. 6 | <10 | <10 | 22 975 | 5 569 | <10 | <10 | ND | ND | ||||
| Average, n = 6 | — | — | 16 493 | 5 766 | — | — | — | — | ||||
| SD | — | — | 4934 | 5028 | — | — | — | — | ||||
| IL-8, pg/mL | ||||||||||||
| Exp. 1 | ND | ND | 370 | 125 | 256 | 667 | 2910 | 3523 | ||||
| Exp. 2 | ND | ND | 641 | 239 | 443 | 891 | 3523 | 3523 | ||||
| Exp. 3 | 313 | 514 | 709 | 292 | 2693 | 3821 | 2276 | ND | ||||
| Average, n = 3 | — | — | 573 | 218 | 1130 | 1793 | — | — | ||||
| SD | — | — | 179 | 85 | 1356 | 1760 | — | — | ||||
| IL-12, pg/mL | ||||||||||||
| Exp. 4 | 9 | 19 | 15 | 9 | 328 | 349 | ND | ND | ||||
| Exp. 5 | <5 | 13 | 23 | 37 | 356 | 466 | ND | ND | ||||
. | None . | . | CpG . | . | CD40L + IL-1 . | . | PGN . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | Del1 . | Control . | Del1 . | Control . | Del1 . | Control . | Del1 . | Control . | ||||
| IFN-α, pg/mL | ||||||||||||
| Exp. 1 | ND | ND | 15 238 | <10 | <10 | <10 | <10 | <10 | ||||
| Exp. 2 | ND | ND | 18 163 | 8 463 | <10 | <10 | <10 | <10 | ||||
| Exp. 3 | <10 | <10 | 20 475 | 60 | <10 | <10 | 50 | ND | ||||
| Exp. 4 | 55 | <10 | 10 655 | 7 700 | <10 | <10 | ND | ND | ||||
| Exp. 5 | <10 | 85 | 11 450 | 12 805 | <10 | <10 | ND | ND | ||||
| Exp. 6 | <10 | <10 | 22 975 | 5 569 | <10 | <10 | ND | ND | ||||
| Average, n = 6 | — | — | 16 493 | 5 766 | — | — | — | — | ||||
| SD | — | — | 4934 | 5028 | — | — | — | — | ||||
| IL-8, pg/mL | ||||||||||||
| Exp. 1 | ND | ND | 370 | 125 | 256 | 667 | 2910 | 3523 | ||||
| Exp. 2 | ND | ND | 641 | 239 | 443 | 891 | 3523 | 3523 | ||||
| Exp. 3 | 313 | 514 | 709 | 292 | 2693 | 3821 | 2276 | ND | ||||
| Average, n = 3 | — | — | 573 | 218 | 1130 | 1793 | — | — | ||||
| SD | — | — | 179 | 85 | 1356 | 1760 | — | — | ||||
| IL-12, pg/mL | ||||||||||||
| Exp. 4 | 9 | 19 | 15 | 9 | 328 | 349 | ND | ND | ||||
| Exp. 5 | <5 | 13 | 23 | 37 | 356 | 466 | ND | ND | ||||
Cells produced on OP9-Del1 (Del1) or OP9-C stroma (control) after 3 weeks of culture from CD34+ cells were collected and stimulated for 24 to 48 hours with the indicated agents to induce the production of cytokines in the culture medium. IFN-α, IL-8, and IL-12 were measured by ELISA.
Exp. indicates experiment; ND, not done; and —, not applicable.