Table 1. Serum levels of autoantibodies... | American Society of Hematology

Table 1.

Serum levels of autoantibodies of BALB/c recipients on day 15 after donor-cell injection

Donor cells, 50 × 10⁶	Total IgG1, μg/mL	Total IgG2a, μg/mL	Anti-dsDNA IgG1, U/mL	Anti-dsDNA IgG2a, U/mL	Anti-dsDNA IgG2a^a, U/mL
DBA/2 whole SPL	1157 ± 58	4033 ± 279	3162 ± 603	1459 ± 163	<10
DBA/2 CD4^--SPL	484 ± 41	731 ± 60	407 ± 43	335 ± 41	<10
DBA/2 CD8^--SPL	1953 ± 204	5051 ± 729	2630 ± 276	1073 ± 481	<10
DBA/2 B220^--SPL	346 ± 43	455 ± 42	225 ± 46	140 ± 16	<10
No injection	97 ± 5	43 ± 4.2	<10	<10	<10
NZB SPL^*	NA	NA	NA	366 ± 79	293 ± 32

Donor cells, 50 × 10⁶	Total IgG1, μg/mL	Total IgG2a, μg/mL	Anti-dsDNA IgG1, U/mL	Anti-dsDNA IgG2a, U/mL	Anti-dsDNA IgG2a^a, U/mL
DBA/2 whole SPL	1157 ± 58	4033 ± 279	3162 ± 603	1459 ± 163	<10
DBA/2 CD4^--SPL	484 ± 41	731 ± 60	407 ± 43	335 ± 41	<10
DBA/2 CD8^--SPL	1953 ± 204	5051 ± 729	2630 ± 276	1073 ± 481	<10
DBA/2 B220^--SPL	346 ± 43	455 ± 42	225 ± 46	140 ± 16	<10
No injection	97 ± 5	43 ± 4.2	<10	<10	<10
NZB SPL^*	NA	NA	NA	366 ± 79	293 ± 32

Data are presented as means ± SE; n = 10.

CD4^--SPL indicates CD4⁺ T cell–depleted spleen cells; CD8^--SPL, CD8⁺ T cell–depleted spleen cells; B220^--SPL, B220⁺ B cell–depleted spleen cells; and NA, not applicable.

IgG2a antibodies secreted by B cells from NZB mice are allotype a, and serum of BALB/c recipients given donor NZB spleen cells was used as positive control for anti-dsDNA IgG2a^a

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