Hemoglobin properties before and after oxygenation
Property . | Deoxygenated . | Oxygenated . |
|---|---|---|
| Fe-NO Hb, μM | 3.7 ± 0.2 | 4.3 ± 0.5 |
| SNO-Hb, μM | 0.66 ± 0.08 | 0.56 ± 0.10 |
| HbO2/total Hb, % | 0.1 ± 0.2 | 97.2 ± 1.6 |
| DeoxyHb/total Hb, % | 99.4 ± 0.2 | 2.8 ± 1.6 |
| MetHb/total Hb, % | 0.6 ± 0.2 | 0.0 ± 0.0 |
| β-Nitrosyl Hb*, % | 50 ± 3 | 45 ± 6 |
| Pentacoordinate α-nitrosyl Hb*, % | 24 ± 3 | 0.3 ± 0.4 |
Property . | Deoxygenated . | Oxygenated . |
|---|---|---|
| Fe-NO Hb, μM | 3.7 ± 0.2 | 4.3 ± 0.5 |
| SNO-Hb, μM | 0.66 ± 0.08 | 0.56 ± 0.10 |
| HbO2/total Hb, % | 0.1 ± 0.2 | 97.2 ± 1.6 |
| DeoxyHb/total Hb, % | 99.4 ± 0.2 | 2.8 ± 1.6 |
| MetHb/total Hb, % | 0.6 ± 0.2 | 0.0 ± 0.0 |
| β-Nitrosyl Hb*, % | 50 ± 3 | 45 ± 6 |
| Pentacoordinate α-nitrosyl Hb*, % | 24 ± 3 | 0.3 ± 0.4 |
Total Hb = 5.2 ± 0.1 mM. Total NO/Hb = 0.00085 ± 0.00005. Data shown as average of 7 trials from 2 samples deoxygenated on different days ± SD. Percentage of hexacoordinate α-nitrosyl Hb is equal to that of 100% minus the sum of the β-nitrosyl Hb and pentacoordinate α-nitrosyl Hb.
Compared to total nitrosyl Hb.