The effect of SLAM signaling on the cytokine production of DCs activated by CD40L, LPS/IFN-γ, or with the mixture of CD40L and LPS/IFN-γ
. | CD40L . | LPS/IFN-γ . | CD40L + LPS/IFN-γ . |
|---|---|---|---|
| IL-12 | |||
| n | 18 | 13 | 10 |
| Production range, ng/mL* (median) | 0.5-56.2 (2.7) | 0.3-26.3 (6.1) | 1.0-146.1 (61.2) |
| SLAM effect, average ± SD† | 0.2 ± 0.1 | 2.3 ± 1.4 | 0.5 ± 0.3 |
| P | < .001 | .005 | .01 |
| TNF-α | |||
| n | 5 | 8 | 6 |
| Production range, ng/mL* (median) | 18-100.1 (52.7) | 0.8-35.0 (11.7) | 78.5-133.7 (99.4) |
| SLAM effect, average ± SD† | 0.2 ± 0.2 | 2.4 ± 1.8 | 0.6 ± 0.1 |
| P | .03 | .04 | .02 |
| IL-6 | |||
| n | 5 | 6 | |
| Production range, ng/mL* (median) | 54.3-197.0 (122.3) | 30.3-140.2 (68.0) | 98.7-209.0 (176.5) |
| SLAM effect, average ± SD† | 0.4 ± 0.1 | 1.3 ± 0.6 | 0.6 ± 0.4 |
| P | .03 | .09 | .02 |
. | CD40L . | LPS/IFN-γ . | CD40L + LPS/IFN-γ . |
|---|---|---|---|
| IL-12 | |||
| n | 18 | 13 | 10 |
| Production range, ng/mL* (median) | 0.5-56.2 (2.7) | 0.3-26.3 (6.1) | 1.0-146.1 (61.2) |
| SLAM effect, average ± SD† | 0.2 ± 0.1 | 2.3 ± 1.4 | 0.5 ± 0.3 |
| P | < .001 | .005 | .01 |
| TNF-α | |||
| n | 5 | 8 | 6 |
| Production range, ng/mL* (median) | 18-100.1 (52.7) | 0.8-35.0 (11.7) | 78.5-133.7 (99.4) |
| SLAM effect, average ± SD† | 0.2 ± 0.2 | 2.4 ± 1.8 | 0.6 ± 0.1 |
| P | .03 | .04 | .02 |
| IL-6 | |||
| n | 5 | 6 | |
| Production range, ng/mL* (median) | 54.3-197.0 (122.3) | 30.3-140.2 (68.0) | 98.7-209.0 (176.5) |
| SLAM effect, average ± SD† | 0.4 ± 0.1 | 1.3 ± 0.6 | 0.6 ± 0.4 |
| P | .03 | .09 | .02 |
n indicates the number of donors tested.
Production range was calculated in samples activated in the absence of SLAM-SLAM interactions.
SLAM effect was calculated by the following formula: cytokine concentration in the presence of SLAM/SLAM interactions/cytokine concentration in the absence of SLAM-SLAM interaction. P values were calculated with signed-rank test or paired t test according to the distribution of variables.