Presenting immunophenotypes before transplantation
| Subject/day of life (day before transplant) . | CD3+/mm3 . | CD4+/mm3 . | CD8+/mm3 . | CD3+ CD4−CD8−/mm3 . | Naive CD4+, %* . | CD19/mm3 . | CD16+ and/or CD56+, CD3−/mm3 . | PHA (bkg), cpm† . | Eosinophils/mm3 . |
|---|---|---|---|---|---|---|---|---|---|
| Subject 1 | |||||||||
| 166 (−258) | 2219 | 660 | 612 | 678 | < 1 | 3940 | 51 | 53 | |
| 215 (−209) | 6250 | 1950 | 1737 | 1507 | < 1 | 1507 | 886 | 4189 (654) | 4155 |
| 254 (−170) | 2296 | 745 | 576 | 749 | < 1 | 1307 | 487 | 2582 (584) | 2436 |
| 297 (−127) | 1463 | 446 | 246 | 752 | < 1 | 1946 | 1667 | 18 912 (103) | 1613 |
| 408 (−16) | 964 | 404 | 146 | 411 | < 2 | 3632 | 582 | 63 733 (413) | 468 |
| 418 (−6) | 1010 | 473 | 155 | 381 | < 1 | 2734 | 409 | 104 246 (154) | 510 |
| Subject 2 | |||||||||
| 140 (−126) | 2‡ | 315 | 273 | ||||||
| 252 (−14) | 1‡ | 638 | 167 | 36 | |||||
| 255 (−11) | 635 (205) | 76 | |||||||
| Subject/day of life (day before transplant) . | CD3+/mm3 . | CD4+/mm3 . | CD8+/mm3 . | CD3+ CD4−CD8−/mm3 . | Naive CD4+, %* . | CD19/mm3 . | CD16+ and/or CD56+, CD3−/mm3 . | PHA (bkg), cpm† . | Eosinophils/mm3 . |
|---|---|---|---|---|---|---|---|---|---|
| Subject 1 | |||||||||
| 166 (−258) | 2219 | 660 | 612 | 678 | < 1 | 3940 | 51 | 53 | |
| 215 (−209) | 6250 | 1950 | 1737 | 1507 | < 1 | 1507 | 886 | 4189 (654) | 4155 |
| 254 (−170) | 2296 | 745 | 576 | 749 | < 1 | 1307 | 487 | 2582 (584) | 2436 |
| 297 (−127) | 1463 | 446 | 246 | 752 | < 1 | 1946 | 1667 | 18 912 (103) | 1613 |
| 408 (−16) | 964 | 404 | 146 | 411 | < 2 | 3632 | 582 | 63 733 (413) | 468 |
| 418 (−6) | 1010 | 473 | 155 | 381 | < 1 | 2734 | 409 | 104 246 (154) | 510 |
| Subject 2 | |||||||||
| 140 (−126) | 2‡ | 315 | 273 | ||||||
| 252 (−14) | 1‡ | 638 | 167 | 36 | |||||
| 255 (−11) | 635 (205) | 76 | |||||||
bkg indicates medium-plus-cells background.
For subject 1, naive T cells were defined by the phenotype CD4+CD45RA+CD27+ for the first 3 values done in the referring center and by the phenotype CD4+CD45RA+CD62L+ in the transplant center. Naive CD8 cells (unpublished) were similarly very low.
The first 2 PHA assays for subject 1 were performed in the referring center; the lower limit of normal is 20 000 counts per minute (cpm). The remaining PHA assays in this table were performed at the transplantation center laboratory in which the lower limit of normal is 75 000 cpm.
The T-cell numbers were too low for accurate determination of the naive percentages.