Quantification of BMD-EC-positive perfused tumor vessels using GFP-reporter mice
. | . | Percent GFP+ functional vessels . | . | |
|---|---|---|---|---|
| Tumor type . | Implantation site . | WT/Actb-Gfp-BMT (C57BL6)* . | WT/Tie2-Gfp-BMT (FVB)† . | |
| Lung adenocarcinoma | Subcutaneous | < 1%‡ (Lewis lung carcinoma) | 4.5 ± 1.6‡ (LA-P0297) | |
| Lung adenocarcinoma metastasis | Lung§ | < 1%∥ (Lewis lung carcinoma) | 10.6 ± 3.6∥¶ (LA-P0297) | |
| Melanoma | Subcutaneous | < 1%‡ (B16) | N/A | |
| Mammary carcinoma | Mammary fat pad/subcutaneous | N/A | 1.5 ± 0.3# (TG1-1)/1.3 ± 0.1# (MCa8) | |
| Mammary carcinoma metastasis | Brain | N/A | 58.4 ± 8.4#** (TG1-1) | |
. | . | Percent GFP+ functional vessels . | . | |
|---|---|---|---|---|
| Tumor type . | Implantation site . | WT/Actb-Gfp-BMT (C57BL6)* . | WT/Tie2-Gfp-BMT (FVB)† . | |
| Lung adenocarcinoma | Subcutaneous | < 1%‡ (Lewis lung carcinoma) | 4.5 ± 1.6‡ (LA-P0297) | |
| Lung adenocarcinoma metastasis | Lung§ | < 1%∥ (Lewis lung carcinoma) | 10.6 ± 3.6∥¶ (LA-P0297) | |
| Melanoma | Subcutaneous | < 1%‡ (B16) | N/A | |
| Mammary carcinoma | Mammary fat pad/subcutaneous | N/A | 1.5 ± 0.3# (TG1-1)/1.3 ± 0.1# (MCa8) | |
| Mammary carcinoma metastasis | Brain | N/A | 58.4 ± 8.4#** (TG1-1) | |
Percent values are shown ± SD obtained after counting GFP+ vessels for different mice (in at least 5 random regions of interest in 5 nonconsecutive sections) in frozen tumor tissue.
N/A indicates not available.
C57BL6 mice that received a bone marrow transplant from Actb-Gfp/C57BL6 mice following lethal irradiation.
FVB mice that received a bone marrow transplant from Tie2-Gfp/FVB mice following lethal irradiation.
Experimental group size was n = 3 mice.
Spontaneous metastases analyzed after primary tumor resection.
Experimental group size was n = 6 mice.
The percentage of BMD-EC-positive vessels was significantly increased in lung metastases in FVB mice compared with primary subcutaneous tumors in FVB mice (P < .05).
Experimental group size was n = 4 mice.
The percentage of BMD-EC-positive vessels was highly significantly increased in mammary carcinoma isografted in brain compared with mammary pad isografts in FVB mice (P < .01).