Switch mutation analyses of WM and IgM MGUS
Group . | No. of sequences . | No. of nucleotides sequenced . | No. of mutations seen . | No. of mutated sequences/total sequences . | Mutation*frequency, × 10-3 bp . |
|---|---|---|---|---|---|
| WM‡ | 11 | 17875 | 10 | 7/11 | 0.56† |
| IgM MGUS | 4 | 6500 | 16 | 3/4 | 2.5† |
Group . | No. of sequences . | No. of nucleotides sequenced . | No. of mutations seen . | No. of mutated sequences/total sequences . | Mutation*frequency, × 10-3 bp . |
|---|---|---|---|---|---|
| WM‡ | 11 | 17875 | 10 | 7/11 | 0.56† |
| IgM MGUS | 4 | 6500 | 16 | 3/4 | 2.5† |
The fidelity of high-fidelity Taq polymerase was determined to be less than 1/10 000 bp. The error rate has been taken into consideration when calculating the significance level of the P value
P = .001; calculated by the Fisher exact test
WM1-01 clone containing 24-bp deletion in the switch region is not included in this analysis