Table 3. Combination of primer sets for... | American Society of Hematology

Table 3.

Combination of primer sets for single-cell heminested RT-PCR

	PCR primers, sense/antisense
Transcript type	First-stage PCR	Second-stage PCR
AID 5′	AID 5′/AID 3′	AID 5′/AID 3′int
AID 3′	AID 5′/AID 3′	AID 5′int/AID 3′
Clonotypic	FRIc/Cμ	CDR2/CDR3
β₂m, control	β₂m 5′/β₂m 3′	β₂m 5′int/β₂m 3′

	PCR primers, sense/antisense
Transcript type	First-stage PCR	Second-stage PCR
AID 5′	AID 5′/AID 3′	AID 5′/AID 3′int
AID 3′	AID 5′/AID 3′	AID 5′int/AID 3′
Clonotypic	FRIc/Cμ	CDR2/CDR3
β₂m, control	β₂m 5′/β₂m 3′	β₂m 5′int/β₂m 3′