Summary of PCR primers
RT-PCR and primer . | Sequence . |
|---|---|
| AID | |
| AID 5′ | 5′-GAGGCAAGAAGACACTCTGG-3′ |
| AID 3′ | 5′-GTGACATTCCTGGAAGTTGC-3′ |
| AID 5′int | 5′-CAACCTCAGTCTGAGGATCTTC-3′ |
| AID 3′int | 5′-GAAGATCCTCAGACTGAGGTTG-3′ |
| Clonotypic VDJ, clonotypic isotypes, repertoire analysis | |
| FR1c | 5′-GGTGCAGCTG (G/C) (A/T) G (G/C) AGTC (G/A/T) GG-3′ |
| JHc | 5′-ACCTGAGGAGACGGTGACC (A/G) (G/T) (G/T) GT-3′ |
| FR3 | 5′-CCGAGGACACGGC (T/C) (C/G) TGTATTACTG-3′ |
| Cμ | 5′-GGAATTCTCACAGGAGAC-3′ |
| Cδ | 5′-GTGTCTGCACCCTGATAT-3′ |
| Cγ | 5′-GGGGAAGACCGATGGGCCCT-3′ |
| Cα | 5′-GAGGCTCAGCGGGAAGACCTT-3′ |
| Internal control | |
| β2m 5′ | 5′-CCAGCAGAGAATGGAAAGTC-3′ |
| β2m 5′int | 5′-TGTCTTTCAGCAAGGACTGG-3′ |
| β2m 3′ | 5′-GATGCTGCTTACATGTCTCG-3′ |
| Switch region analysis | |
| Sw15as | 5′-GAAAGCTGGATGGAGTTGTC-3′ |
| SwA | 5′-CCGCAGGCAGCCAATAGAGT-3′ |
| Sγ | 5′-TCCCAGTGTCCTGCATTACTTC-3′ |
| Germline transcripts | |
| slμ | 5′-GTGATTAAGGAGAAACACTTTGAT-3′ |
| slγ1/2 | 5′-GGGCTTCCAAGCCAACAGGGCAGGACA-3′ |
| slγ3 | 5′-AGGTGGGCAGGCTTCAGGCACCGAT-3′ |
| slγ4 | 5′-TTGTCCAGGCCGGCAGCATCACCAGA-3′ |
| slϵ | 5′-GACGGGCCACACCATCC-3′ |
| asCμ | 5′-CCGAATTCAGACGAGGGGGAAAAGGGTT-3′ |
| asCγ? | 5′-GTTTTGTCACAAGATTTGGGCTC-3′ |
| asCγ2 | 5′-GTGGGCACTCGACACAACATTTGCG-3′ |
| asCγ3 | 5′-TTGTGTCACCAAGTGGGGTTTTGAGC-3′ |
| asCγ4 | 5′-ATGGGCATGGGGGACCATTTGGA-3′ |
| asCϵ | 5′-CGGAGGTGGCATTGGAGG-3′ |
RT-PCR and primer . | Sequence . |
|---|---|
| AID | |
| AID 5′ | 5′-GAGGCAAGAAGACACTCTGG-3′ |
| AID 3′ | 5′-GTGACATTCCTGGAAGTTGC-3′ |
| AID 5′int | 5′-CAACCTCAGTCTGAGGATCTTC-3′ |
| AID 3′int | 5′-GAAGATCCTCAGACTGAGGTTG-3′ |
| Clonotypic VDJ, clonotypic isotypes, repertoire analysis | |
| FR1c | 5′-GGTGCAGCTG (G/C) (A/T) G (G/C) AGTC (G/A/T) GG-3′ |
| JHc | 5′-ACCTGAGGAGACGGTGACC (A/G) (G/T) (G/T) GT-3′ |
| FR3 | 5′-CCGAGGACACGGC (T/C) (C/G) TGTATTACTG-3′ |
| Cμ | 5′-GGAATTCTCACAGGAGAC-3′ |
| Cδ | 5′-GTGTCTGCACCCTGATAT-3′ |
| Cγ | 5′-GGGGAAGACCGATGGGCCCT-3′ |
| Cα | 5′-GAGGCTCAGCGGGAAGACCTT-3′ |
| Internal control | |
| β2m 5′ | 5′-CCAGCAGAGAATGGAAAGTC-3′ |
| β2m 5′int | 5′-TGTCTTTCAGCAAGGACTGG-3′ |
| β2m 3′ | 5′-GATGCTGCTTACATGTCTCG-3′ |
| Switch region analysis | |
| Sw15as | 5′-GAAAGCTGGATGGAGTTGTC-3′ |
| SwA | 5′-CCGCAGGCAGCCAATAGAGT-3′ |
| Sγ | 5′-TCCCAGTGTCCTGCATTACTTC-3′ |
| Germline transcripts | |
| slμ | 5′-GTGATTAAGGAGAAACACTTTGAT-3′ |
| slγ1/2 | 5′-GGGCTTCCAAGCCAACAGGGCAGGACA-3′ |
| slγ3 | 5′-AGGTGGGCAGGCTTCAGGCACCGAT-3′ |
| slγ4 | 5′-TTGTCCAGGCCGGCAGCATCACCAGA-3′ |
| slϵ | 5′-GACGGGCCACACCATCC-3′ |
| asCμ | 5′-CCGAATTCAGACGAGGGGGAAAAGGGTT-3′ |
| asCγ? | 5′-GTTTTGTCACAAGATTTGGGCTC-3′ |
| asCγ2 | 5′-GTGGGCACTCGACACAACATTTGCG-3′ |
| asCγ3 | 5′-TTGTGTCACCAAGTGGGGTTTTGAGC-3′ |
| asCγ4 | 5′-ATGGGCATGGGGGACCATTTGGA-3′ |
| asCϵ | 5′-CGGAGGTGGCATTGGAGG-3′ |
β2m indicates β2-microglobulin.